Generation of Human Blastoids from Naive Pluripotent Stem Cells

  • Methods Mol Biol. 2023 Jun 24. doi: 10.1007/7651_2023_485.
Yulei Wei  1  2 ,  Kun Liu  3  4 ,  Carlos A Pinzon-Arteaga  1 ,  Deirdre Logsdon  3 ,  Leqian Yu  1  5  6 ,  Ye Yuan  3 ,  Jun Wu  7  8  9
Affiliations
  • 1. Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • 2. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
  • 3. Colorado Center for Reproductive Medicine, Lone Tree, CO, USA.
  • 4. Paul M. Rady Department of Mechanical Engineering, University of Colorado, Boulder, CO, USA.
  • 5. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • 6. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
  • 7. Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. [email protected].
  • 8. Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA. [email protected].
  • 9. Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. [email protected].
PMID: 37351840 DOI: 10.1007/7651_2023_485
Abstract

Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.

Keywords
Human blastoids; Human extraembryonic cell types; Human implantation model; Hypoblast cells; Integrated models of human embryos; Naive human pluripotent stem cells; The generation of blastocyst-like structures; Trophoblast cells.
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