1. PI3K/Akt/mTOR
    Stem Cell/Wnt
  2. GSK-3
  3. IM-12


Cat. No.: HY-12292 Purity: 98.30%
Handling Instructions

IM-12 is an inhibitor of GSK-3β, with an IC50 of 53 nM, and also enhances Wnt signalling.

For research use only. We do not sell to patients.

IM-12 Chemical Structure

IM-12 Chemical Structure

CAS No. : 1129669-05-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 108 In-stock
Estimated Time of Arrival: December 31
10 mg USD 98 In-stock
Estimated Time of Arrival: December 31
50 mg USD 362 In-stock
Estimated Time of Arrival: December 31
100 mg USD 610 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE IM-12

    IM-12 purchased from MCE. Usage Cited in: J Cell Physiol. 2019 Jul;234(7):10698-10708.

    Cell migration and invasion assays are conducted using Transwell chambers with the treatment of IM-12.

    View All GSK-3 Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review


    IM-12 is an inhibitor of GSK-3β, with an IC50 of 53 nM, and also enhances Wnt signalling.

    IC50 & Target[1]


    53 nM (IC50)

    In Vitro

    IM-12 inhibits GSK-3β in ReNcell VM cells, with I50 of 3.8 μM. IM-12 (3 μM) enhances the β-catenin amount, with no further effect at lower or higher concentration. IM-12 (3 μM) also attenuates the proliferation of ReNCell VM cells. IM-12 increases TCF-activity of ReNcell VM[1].

    Molecular Weight




    CAS No.





    Room temperature in continental US; may vary elsewhere.

    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 54.9 mg/mL (145.47 mM)

    *"≥" means soluble, but saturation unknown.

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6496 mL 13.2482 mL 26.4964 mL
    5 mM 0.5299 mL 2.6496 mL 5.2993 mL
    10 mM 0.2650 mL 1.3248 mL 2.6496 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    Kinase Assay

    Cells are lysed in RIPA buffer, supplemented with protease and phosphatase inhibitors and centrifuged for 5 min at 15,000 rpm. Immunoprecipitation of GSK-3β is performed with a specific mouse monoclonal anti GSK-3β [G8] antibody with 5 μg/sample for 2 h at 4°C. The bound protein is precipitated with Protein A/G-Plus agarose-beads (10 μL beads per sample). GSK-3β kinase activity is measured in a reaction mixture containing final concentrations of: 4 mM MOPS pH 7.2; 0.4 mM EDTA; 1 mM EGTA; 2.5 mM β-glycerophosphate; 4 mM MgCl2; 40 μM BSA; 0.05 mM DTT. 10 μg/sample pGS-2 peptide substrate is used[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay

    To measure viable cells, 50-100 μL of cell suspension is analyzed using CASY technology with the appropriate program. ReNcell VM cells are seeded at a defined cell number and proliferated for 24 h. Then the medium is changed to proliferation medium with added substances at indicated concentrations. The cell number is determined every 24 h. Cells are exposed to the added drugs during the whole experiment, whereas the media is changed every 24 h[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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    IM-12IM12IM 12GSK-3Glycogen synthase kinase-3Glycogen synthase kinase 3Inhibitorinhibitorinhibit

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