IM-12 

IM-12 GMP is IM-12 (HY-12292) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. IM-12 is an orally active anti-inflammatory agent that targets and inhibits the NF-κB and STAT3 signaling pathways. IM-12 activates the Wnt signaling pathway and promotes the nuclear translocation of β-catenin by inhibiting GSK3β, while also blocking the tyrosine kinase activity of p210BCR/ABL. IM-12 reduces the levels of IL-6, IL-17, NO, prostaglandin E2, iNOS and COX-2, and induces ER stress, Ca²⁺ release, autophagy and apoptosis. IM-12 is heat-sensitive and does not induce autophagy in IM-resistant p210BCR/ABL^T315I mutant cells. IM-12 is also a component of the 5iLA medium used for naive pluripotent stem cell research. IM-12 has been applied in studies related to carrageenan (HY-125474)-induced hind paw edema, TNBS-induced colitis, and acute and chronic myeloid leukemia^{[1][2][3][4][5]}.

IM-12 GMP (1 μM; long-term culture) reduces the proliferation rate of human naive pluripotent stem cells and renders cell colonies more compact in morphology. IM-12 GMP upregulates genes associated with oxidative phosphorylation and cell adhesion, downregulates genes related to development and stem cell differentiation, while maintaining the expression of naive pluripotency markers^[1].
IM-12 GMP also enables cells to spontaneously form blastoids that mimic human early blastocysts in 3D culture, and these blastoids can further simulate the developmental process of post-implantation embryos^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IM-12 GMP (0.2×10⁹-5×10⁹ cfu/mouse; p.o.; daily; 3 days) significantly attenuates carrageenan-induced paw oedema in male ICR mice, with the 1×10⁹ cfu/mouse dose demonstrating the most potent inhibition of inflammatory markers and signalling pathways^[2].
IM-12 GMP (1×10⁹ cfu/mouse; p.o.; daily; 3 days) significantly attenuates TNBS-induced colitis in male C57BL/6 mice by suppressing inflammatory markers and NF-κB-STAT3 signalling, with both live and heat-inactivated forms showing efficacy^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

377.41

C₂₂H₂₀FN₃O₂

1129669-05-1

O=C(C(NCCC1=CC=C(F)C=C1)=C2C3=C(C)NC4=C3C=CC=C4)N(C)C2=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Guo M, Wu J, Chen C, et al. Self-renewing human naïve pluripotent stem cells dedifferentiate in 3D culture and form blastoids spontaneously[J]. Nature communications, 2024, 15(1): 668.

  [2]. Lim SM, et al. Lactobacillus fermentum IM12 attenuates inflammation in mice by inhibiting NF-κB-STAT3 signalling pathway. Benef Microbes. 2017;8(3):407-419.

  [3]. Semi K, et al. Pluripotent stem cells for the study of early human embryology. Dev Growth Differ. 2021;63(2):104-115.

  [4]. Kikushige Y, et al. A TIM-3/Gal-9 Autocrine Stimulatory Loop Drives Self-Renewal of Human Myeloid Leukemia Stem Cells and Leukemic Progression. Cell Stem Cell. 2015;17(3):341-352.

  [5]. Bellodi C, et al. Targeting autophagy potentiates tyrosine kinase inhibitor-induced cell death in Philadelphia chromosome-positive cells, including primary CML stem cells. J Clin Invest. 2009;119(5):1109-1123.