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CD20 Antibody (YA1303)

(Synonyms: Bp35, MS4A1)
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Based on 1 Customer Validation

CD20 Antibody (YA1303) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD20.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD20 Antibody (YA1303)
  • Experimental Validation Results for CD20 Antibody (YA1303)
  • CD20 Antibody (YA1303) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100

Product Details

Description

CD20 Antibody (YA1303) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD20.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size:33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database

Entrez Gene: 931 Human

SwissProt: P11836 Human

Immunogen

A synthesized peptide derived from human CD20 aa250-297.

Sensitivity

Endogenous

Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103670

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD20 Antibody (YA1303)
    Western blot analysis of extracts from Ramos(lane 2, 20μg), Raji(lane 3, 20μg), Daudi(lane 4, 20μg) and HeLa(lane 5, 20μg) using CD20 Antibody (HY-P81558). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD20 Antibody (YA1303)
    Flow cytometric analysis of 1X10^6 Raji cells labeling CD20 Antibody (HY-P81558, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD20 is a 33-37 kDa non-glycosylated protein expressed specifically on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 is a general B-cell marker expressed by the majority of B cells starting from late pre-B lymphocytes (it is not expressed by pro-B lymphocytes), and its expression is lost in terminally differentiated plasmablasts and plasma cells. Early experiments suggested that it was important in the regulation of human B-cell activation, proliferation, and Ca2+ transport. However, CD20-deficient mice appeared normal. Hence, the exact biological function of CD20 and its physiological ligand remain to be fully defined.240 Regardless of the uncertainty in the biological role of this antigen, it is a good target for cancer immunotherapy.

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Cell membrane; Lipid-anchor

  • Expression


    Tissue_specificity:Expressed on B cells

  • Isoforms & Post-Translational Modification

    CD20 has 2 isomers: P11836-1: 33077 Da (predicted); P11836-2: 14581 Da (predicted).
    Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate

  • Subunit

    Forms homotetramers (PubMed:18474602, PubMed:7684739). Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR (PubMed:18474602)

  • SwissProt ID

    P11836

  • Gene ID
    931 [NCBI]
  • Synonyms

    Bp35, MS4A1

  • Research Field

    Cell biology

CD20 Antibody (YA1303) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Ramos(lane 2, 20μg), Raji(lane 3, 20μg), Daudi(lane 4, 20μg) and HeLa(lane 5, 20μg) using CD20 Antibody (HY-P81558). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Raji cells labeling CD20 Antibody (HY-P81558, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD20 Antibody (YA1303) Product Image
    1/16
  • Experimental Validation Results for CD20 Antibody (YA1303)
  • Experimental Validation Results for CD20 Antibody (YA1303)
  • CD20 Antibody (YA1303) Product Image
  • Experimental Validation Results for CD20 Antibody (YA1303)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Ramos(lane 2, 20μg), Raji(lane 3, 20μg), Daudi(lane 4, 20μg) and HeLa(lane 5, 20μg) using CD20 Antibody (HY-P81558). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD20 Antibody (YA1303)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Raji cells labeling CD20 Antibody (HY-P81558, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD20 Antibody (YA1303)
  • Experimental Validation Results for CD20 Antibody (YA1303)
  • CD20 Antibody (YA1303) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Ramos(lane 2, 20μg), Raji(lane 3, 20μg), Daudi(lane 4, 20μg) and HeLa(lane 5, 20μg) using CD20 Antibody (HY-P81558). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • CD20 Antibody (YA1303) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Raji cells labeling CD20 Antibody (HY-P81558, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD20 Antibody (YA1303) Product Image WB
  • CD20 Antibody (YA1303) Product Image FC