S1PR4 Antibody
(Synonyms: EDG6; LPC1; S1P4; SLP4)S1PR4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to S1PR4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Rat
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Formulation:
Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
S1PR4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to S1PR4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityRat
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 42 kDa
Entrez Gene: 314649 Rat
Endogenous
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
S1PR4 is an S1P-responsive G-protein-coupled receptor that regulates cell shape and motility through Gαi and Gα12/13 coupling[1]. In immune cells, S1PR4 transduces S1P effects on T-cell proliferation and cytokine secretion without signaling migration, distinguishing it from migration-dominant S1P receptor functions[2]. Mechanistically, S1PR4 deficiency affects dendritic-cell function and Th17-cell differentiation in a murine model[3]. S1PR4 is also required for plasmacytoid dendritic-cell differentiation, because S1PR4-deficient mice show reduced plasmacytoid dendritic-cell abundance across organs[4]. In human plasmacytoid dendritic cells, S1PR4 signaling preserves ILT7 surface expression and limits TLR7/9-induced IFN-α production[5]. In disease models, S1PR4 promotes nonalcoholic steatohepatitis by activating the NLRP3 inflammasome in hepatic macrophages[6]. Compared with related isoforms, S1PR4 shows a functional profile centered on immune-cell activation, cytokine control, dendritic-cell biology, and inflammasome-linked macrophage responses rather than broad chemotactic signaling[2][3][5][6]. For experimental applications, potent and selective S1P4 receptor agonists provide pharmacological tools to decipher S1P4 biological function and assess therapeutic utility[7].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Specifically expressed in human lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in human spleen as well as human peripheral leukocytes and lung. Lower expression in human thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung -
SwissProt ID
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Synonyms
EDG6; LPC1; S1P4; SLP4
Documentation
[1]. Gräler MH, et al. The sphingosine 1-phosphate receptor S1P4 regulates cell shape and motility via coupling to Gi and G12/13. J Cell Biochem. 2003 Jun 1;89(3):507-19. [Content Brief]
[2]. Wang W, et al. Type 4 sphingosine 1-phosphate G protein-coupled receptor (S1P4) transduces S1P effects on T cell proliferation and cytokine secretion without signaling migration. FASEB J. 2005 Oct;19(12):1731-3. [Content Brief]
[3]. Schulze T, et al. Sphingosine-1-phospate receptor 4 (S1P₄) deficiency profoundly affects dendritic cell function and TH17-cell differentiation in a murine model. FASEB J. 2011 Nov;25(11):4024-36. [Content Brief]
[4]. Dillmann C, et al. S1PR4 is required for plasmacytoid dendritic cell differentiation. Biol Chem. 2015 Jun;396(6-7):775-82. [Content Brief]
[5]. Dillmann C, et al. S1PR4 Signaling Attenuates ILT 7 Internalization To Limit IFN-α Production by Human Plasmacytoid Dendritic Cells. J Immunol. 2016 Feb 15;196(4):1579-90. [Content Brief]
[6]. Hong CH, et al. Sphingosine 1-Phosphate Receptor 4 Promotes Nonalcoholic Steatohepatitis by Activating NLRP3 Inflammasome. Cell Mol Gastroenterol Hepatol. 2022;13(3):925-947. [Content Brief]
[7]. Guerrero M, et al. Discovery, design and synthesis of novel potent and selective sphingosine-1-phosphate 4 receptor (S1P₄-R) agonists. Bioorg Med Chem Lett. 2012 Jan 1;22(1):537-42. [Content Brief]
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