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Cat. No.: HY-P84097A

Cyclin B1 Antibody (YA3794)(PBS only)

Name: Cyclin B1 Antibody (YA3794)(PBS only)
Brand: MedChemExpress
SKU: HY-P84097A
Availability: PreOrder

(Synonyms: CCNB; CCNB1)

Cyclin B1 Antibody (YA3794) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cyclin B1.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	FC FC: Flow Cytometry	ELISA ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio	1:500-1:2000	1:200-1:1000	1:200-1:400	1:10000

Description

Cyclin B1 Antibody (YA3794) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cyclin B1.

Host Mouse
Species Reactivity
Human, Rat
Observed Molecular Weight

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 48 kDa

Immunogen

Purified recombinant fragment of human CCNB1 aa 1-197.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG1

Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition
Subcellular Localization

Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Isoforms & Post-Translational Modification

P14635 has 2 isomers: P14635-1: 48337 Da (predicted); P14635-2: 44102 Da (predicted).
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811);Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site
Subunit

Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3 (PubMed:15790566). Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1 (PubMed:21540187)
SwissProt ID

P14635
Gene ID

891 [NCBI]
Synonyms

CCNB; CCNB1