ERK8 Antibody (YA6556)

Cat. No.: HY-P86863: Data Sheet COA
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ERK8 Antibody (YA6556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK8.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ERK8 Antibody (YA6556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK8.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 60 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8TD08

Gene ID

225689 [NCBI]

Immunogen

Recombinant protein within human ERK8 aa 1-400.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:20733054, PubMed:21847093, PubMed:22948227, PubMed:24618899, PubMed:29021280). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:19166846, PubMed:20638370). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936)

Subcellular Localization：Cytoplasm, cytoskeleton, cilium basal body,Cell junction, tight junction,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole,Cytoplasmic vesicle, autophagosome,Golgi apparatus,Nucleus,Cytoplasm,Cytoplasm, cytoskeleton, spindle

Expression：
Tissue_Specificity: Widely expressed with a maximal expression in lung and kidney

Isoforms & Post-Translational Modification：Q8TD08 has 3 isomers: Q8TD08-1: 59832 Da (predicted); Q8TD08-2: 28696 Da (predicted); Q8TD08-3: 31112 Da (predicted).
Autophosphorylated on Thr-175 and Tyr-177; activates the enzyme;Ubiquitinated (PubMed:19166846). Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase (By similarity)

Subunit：Interacts with CSK/c-Src, ABL1, RET and TGFB1I1 (PubMed:11875070, PubMed:16484222, PubMed:16624805)

Synonyms

ERK 7 antibody; ERK 8 antibody; ERK-7 antibody; ERK-8 antibody; ERK7 antibody; ERK8 antibody; Extracellular regulated kinase 8 delta antibody; Extracellular signal regulated kinase 8 antibody; Extracellular signal-regulated kinase 7 antibody; Extracellular signal-regulated kinase 8 antibody; ERK 7 antibody; ERK 8 antibody; ERK-7 antibody; ERK-8 antibody; ERK7 antibody; ERK8 antibody; Extracellular regulated kinase 8 delta antibody; Extracellular signal regulated kinase 8 antibody; Extracellular signal-regulated kinase 7 antibody; Extracellular signal-regulated kinase 8 antibody; MAP kinase 15 antibody; MAPK 15 antibody; Mapk15 antibody; Mitogen activated protein kinase 15 antibody; Mitogen-activated protein kinase 15 antibody; MK15_HUMAN antibody;

Documentation

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Data Sheet (231 KB) SDS (253 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)