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ERK8 Antibody (YA6556)

(Synonyms: ERK 7 antibody; ERK 8 antibody; ERK-7 antibody; ERK-8 antibody; ERK7 antibody; ERK8 antibody; Extracellular regulated kinase 8 delta antibody; Extracellular signal regulated kinase 8 antibody; Extracellular signal-regulated kinase 7 antibody; Extracellular signal-regulated kinase 8 antibody; ERK 7 antibody; ERK 8 antibody; ERK-7 antibody; ERK-8 antibody; ERK7 antibody; ERK8 antibody; Extracellular regulated kinase 8 delta antibody; Extracellular signal regulated kinase 8 antibody; Extracellular signal-regulated kinase 7 antibody; Extracellular signal-regulated kinase 8 antibody; MAP kinase 15 antibody; MAPK 15 antibody; Mapk15 antibody; Mitogen activated protein kinase 15 antibody; Mitogen-activated protein kinase 15 antibody; MK15_HUMAN antibody; )
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ERK8 Antibody (YA6556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • ERK8 Antibody (YA6556) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:200-1:1000

Product Details

Description

ERK8 Antibody (YA6556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Immunogen

Recombinant protein within human ERK8 aa 1-400.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    ERK8, also known as MAPK15, is an atypical mitogen-activated protein kinase that regulates autophagy, ciliogenesis, protein trafficking, and genome integrity through mechanisms that differ from those of classical ERK family members[1][2]. Mechanistically, ERK8 participates in the autophagic machinery by interacting with LC3 and GABARAP family proteins and by regulating ULK1 complex activity, thereby promoting early stages of autophagosome biogenesis and cellular stress adaptation[3][4]. In addition to its role in autophagy, ERK8 functions as a chromatin-associated kinase that preserves genomic stability by preventing HDM2-mediated degradation of proliferating cell nuclear antigen (PCNA), a key factor required for DNA replication and DNA damage protection[5]. Disease-related studies have linked MAPK15 dysregulation to multiple cancer models, including male germ cell tumors, ovarian cancer, gastric cancer, and lung adenocarcinoma, where elevated MAPK15 expression has been associated with tumor cell proliferation, migration, or metastatic behavior[6][7]. Compared with the canonical ERK1/2 isoforms that primarily operate within the Ras-Raf-MEK-ERK signaling cascade, ERK8 is classified as an atypical MAP kinase and exhibits distinct regulatory mechanisms and substrate interactions, supporting specialized functions in autophagy, genome maintenance, and ciliary biology[1][2]. For experimental applications, structural characterization of the ERK8 kinase domain has provided a framework for target validation and future development of selective pharmacological modulators for mechanistic studies[8].

  • Subcellular Localization

    Cytoplasm, cytoskeleton, cilium basal body,Cell junction, tight junction,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole,Cytoplasmic vesicle, autophagosome,Golgi apparatus,Nucleus,Cytoplasm,Cytoplasm, cytoskeleton, spindle

  • Expression


    Tissue_Specificity: Widely expressed with a maximal expression in lung and kidney

  • Isoforms & Post-Translational Modification

    Q8TD08 has three isomers: Q8TD08-1: 59832 Da (predicted); Q8TD08-2: 28696 Da (predicted); Q8TD08-3: 31112 Da (predicted).
    Autophosphorylated on Thr-175 and Tyr-177; activates the enzyme丨Ubiquitinated (PubMed:19166846)

  • Subunit

    Interacts with CSK/c-Src, ABL1, RET and TGFB1I1 (PubMed:11875070, PubMed:16484222, PubMed:16624805)

  • SwissProt ID

    Q8TD08

  • Gene ID
    225689 [NCBI]
  • Synonyms

    ERK 7 antibody; ERK 8 antibody; ERK-7 antibody; ERK-8 antibody; ERK7 antibody; ERK8 antibody; Extracellular regulated kinase 8 delta antibody; Extracellular signal regulated kinase 8 antibody; Extracellular signal-regulated kinase 7 antibody; Extracellular signal-regulated kinase 8 antibody; ERK 7 antibody; ERK 8 antibody; ERK-7 antibody; ERK-8 antibody; ERK7 antibody; ERK8 antibody; Extracellular regulated kinase 8 delta antibody; Extracellular signal regulated kinase 8 antibody; Extracellular signal-regulated kinase 7 antibody; Extracellular signal-regulated kinase 8 antibody; MAP kinase 15 antibody; MAPK 15 antibody; Mapk15 antibody; Mitogen activated protein kinase 15 antibody; Mitogen-activated protein kinase 15 antibody; MK15_HUMAN antibody;

References

[1]. Uniprotkb.

[2]. ERK8 gene information from NCBI.

[3]. Colecchia D, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy. 2012 Dec;8(12):1724-40. [Content Brief]

[4]. Colecchia D, et al. MAPK15 is part of the ULK complex and controls its activity to regulate early phases of the autophagic process. J Biol Chem. 2018 Oct 12;293(41):15962-15976. [Content Brief]

[5]. Groehler AL, et al. A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA. J Cell Biol. 2010 Aug 23;190(4):575-86. [Content Brief]

[6]. Yu FY, et al. The Atypical MAP Kinase MAPK15 Is Required for Lung Adenocarcinoma Metastasis via Its Interaction with NF-κB p50 Subunit and Transcriptional Regulation of Prostaglandin E2 Receptor EP3 Subtype. Cancers (Basel). 2023 Feb 22;15(5):1398. [Content Brief]

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ERK8 Antibody (YA6556) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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