Aquaporin 4 Antibody

Cat. No.: HY-P83459A: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Aquaporin 4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 4.

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사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Aquaporin 4 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

Aquaporin 4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 4.

Host

Rabbit

Clonality

Polyclonal

분자량

Predicted band size: 36 kDa;

Observed band size:34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Rabbit,Pig,Cow,Sheep

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P55088

Gene ID

361 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human AQP4 271-323/323

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
FC FC: Flow Cytometry	1μg/test
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Aquaporin 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Aquaporin 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Forms a water-specific channel (PubMed:18286643, PubMed:8660998, PubMed:9276712). Plays an important role in brain water homeostasis and in glymphatic solute transport (PubMed:22896675, PubMed:27751903, PubMed:30557661, PubMed:30561329). Required for a normal rate of water exchange across the blood brain interface (PubMed:30557661). Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP (PubMed:22896675, PubMed:27751903, PubMed:30561329). Plays a redundant role in urinary water homeostasis and urinary concentrating ability (PubMed:9276712)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Endosome membrane; Cell membrane, sarcolemma; Multi-pass membrane protein; Cell projection

Expression：
Tissue_specificity:Detected in the cerebral cortex, particularly around cortical blood vessels and beneath the pia mater, with lower concentrations in the parenchymal membrane (PubMed: 27751903) . Detected in ependymal cells and astrocytes (PubMed: 22896675, PubMed: 27751903, PubMed: 8660998, PubMed: 9276712) . Detected in supporting Henson cells, internal sulcus cells, and Claudius cells of the inner ear (PubMed: 11406631) . Detected in skeletal muscle (PubMed: 29055082) . Detected in gastric parietal cells (PubMed: 10915655) . Detected in the chief cells of the collecting ducts of the renal medulla (protein level) (PubMed: 16641094, PubMed: 9276712) . Also detected in the brain, heart, and skeletal muscle (PubMed: 8660998) .

Isoforms & Post-Translational Modification：P55088 has 3 isomers: P55088-1: 34436 Da (predicted); P55088-2: 32088 Da (predicted); P55088-3: 37977 Da (predicted).
Phosphorylation by PKC at Ser-180 reduces conductance by 50% (By similarity). Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%; this increase is not due to increased presence at the cell membrane (PubMed:18286643);Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated

Subunit：Homotetramer. The tetramers can form oligomeric arrays in membranes. The size of the oligomers differs between tissues and is smaller in skeletal muscle than in brain. Interaction between AQP4 oligomeric arrays in close-by cells can contribute to cell-cell adhesion (By similarity). Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1 (By similarity)

RRID

AB_3105031

Database

Entrez Gene: 361 Human ; 11829 Mouse ; 25293 Rat

SwissProt: P55087 Human ; P55088 Mouse ; P47863 Rat

OMIM: 620448 Human

Synonyms

aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4.

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Aquaporin 4 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Aquaporin 4 Antibody

Aquaporin 4 Antibody Featured Recommendations:

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Background

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Aquaporin 4 Antibody Related Classifications

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