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Topoisomerase 3 beta1 Antibody

(Synonyms: TOP3B1, TOP3B, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1)

Topoisomerase 3 beta1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Topoisomerase 3 beta1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Topoisomerase 3 beta1 Antibody
  • Topoisomerase 3 beta1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-2000 1:100-200

Product Details

Description

Topoisomerase 3 beta1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Topoisomerase 3 beta1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 96 kDa
Immunogen

Synthetic peptide corresponding to the N-term region of human Topoisomerase 3 beta1.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Topoisomerase 3 beta1 Antibody
    Western blot analysis of extracts from K562 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug) and HepG2 (lane 4, 20ug) using Topoisomerase 3 beta1 Antibody (HY-P810952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Topoisomerase 3 beta1 is a Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity

  • Expression


    Tissue_Specificity: Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.

  • Isoforms & Post-Translational Modification

    Topoisomerase 3 beta1 has 3 isoforms, O95985-1: amino acid length is 862, molecular weight is 96662 Da (predicted); O95985-2: amino acid length is 730, molecular weight is 82123 Da (predicted); O95985-3: amino acid length is 707, molecular weight is 79839 Da (predicted).

  • SwissProt ID

    O95985

  • Gene ID
    8940 [NCBI]
  • Synonyms

    TOP3B1, TOP3B, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1

Topoisomerase 3 beta1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug) and HepG2 (lane 4, 20ug) using Topoisomerase 3 beta1 Antibody (HY-P810952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Topoisomerase 3 beta1 Antibody Product Image
    1/16
  • Experimental Validation Results for Topoisomerase 3 beta1 Antibody
  • Topoisomerase 3 beta1 Antibody Product Image
  • Experimental Validation Results for Topoisomerase 3 beta1 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug) and HepG2 (lane 4, 20ug) using Topoisomerase 3 beta1 Antibody (HY-P810952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Topoisomerase 3 beta1 Antibody
  • Topoisomerase 3 beta1 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug) and HepG2 (lane 4, 20ug) using Topoisomerase 3 beta1 Antibody (HY-P810952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Topoisomerase 3 beta1 Antibody Product Image WB