Sortilin Antibody (YA7547)
Sortilin Antibody (YA7547) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Sortilin.
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Host:
Rabbit
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Isotype:
IgG,Kappa
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
Sortilin Antibody (YA7547) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Sortilin.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 92 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Sortilin functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi[1].
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Subcellular Localization
Golgi apparatus, Golgi stack membrane,Endosome membrane,Endoplasmic reticulum membrane,Nucleus membrane,Cell membrane,Lysosome membrane
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Expression
Tissue_Specificity: Expressed in brain and prostate (at protein level). Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver
Induction: During osteoblast differentiation -
Isoforms & Post-Translational Modification
Q99523 has two isomers: Q99523-1: 92068 Da (predicted); Q99523-2: 77375 Da (predicted).
The N-terminal propeptide is cleaved by furin and possibly other homologous proteases丨Palmitoylated (PubMed:18817523)丨Phosphorylation at Ser-825 facilitates the interaction with GGA1 -
Subunit
Interacts with LPL and SLC2A4 (By similarity)
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SwissProt ID
Documentation
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