Casein Kinase 1 alpha Antibody (YA564)(PBS only)

Cat. No.: HY-P80585A: Data Sheet User Guide for Antibodies
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Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 1 alpha.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 1 alpha.

Host

Rabbit

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P48729

Gene ID

1452 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Casein Kinase 1 alpha.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (PubMed:11955436, PubMed:1409656, PubMed:18305108, PubMed:23902688). It can phosphorylate a large number of proteins (PubMed:11955436, PubMed:1409656, PubMed:18305108, PubMed:23902688). Participates in Wnt signaling (PubMed:11955436). Phosphorylates CTNNB1 at 'Ser-45' (PubMed:11955436). May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis (PubMed:1409656). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688). Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor (PubMed:22017875, PubMed:22017877). Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity)

Subcellular Localization：Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome, centromere, kinetochore; Nucleus speckle; Cytoplasm, cytoskeleton, cilium basal body; Cytoplasm, cytoskeleton, spindle

Isoforms & Post-Translational Modification：P48729 has 3 isomers: P48729-1: 38915 Da (predicted); P48729-2: 41937 Da (predicted); P48729-3: 37567 Da (predicted).
Phosphorylated by MTOR in response to mitogenic stimulation, leading to its activation

Subunit：Interacts with the Axin complex (PubMed:11955436). Interacts with TUT1, leading to TUT1 phosphorylation (PubMed:1409656).

Research Field

Signal Transduction

Synonyms

CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/no-10-cell.html