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RAP1GAP Antibody (YA1826)

(Synonyms: RAP1GAP; KIAA0474; RAP1GA1; Rap1 GTPase-activating protein 1; Rap1GAP; Rap1GAP1)
Customer Review

Based on 1 Customer Validation

RAP1GAP Antibody (YA1826) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP1GAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • RAP1GAP Antibody (YA1826) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

RAP1GAP Antibody (YA1826) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP1GAP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 73 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human RAP1GAP

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104200

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Western blot analysis of extracts from A549(lane 2(20μg), C6(lane 3(20μg), 3T3(lane 4(20μg), HeLa(lane 5(20μg) using RAP1GAP Antibody (HY-P82081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Immunohistochemical analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    RAP1GAP is a GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state

  • Subcellular Localization

    Golgi apparatus membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues

    Induction:By 12-O-tetradecanoylphorbol-13-acetate (TPA) in promyelocytic HL-60 cells

  • Isoforms & Post-Translational Modification

    P47736 has 4 isomers: P47736-1: 73361 Da (predicted); P47736-2: 75187 Da (predicted); P47736-3: 76592 Da (predicted); P47736-4: 80645 Da (predicted).

  • Subunit

    Homodimer and heterodimer with RAP1B

  • SwissProt ID

    P47736

  • Gene ID
    5909 [NCBI]
  • Synonyms

    RAP1GAP; KIAA0474; RAP1GA1; Rap1 GTPase-activating protein 1; Rap1GAP; Rap1GAP1

  • Research Field

    Signal Transduction

RAP1GAP Antibody (YA1826) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg), C6(lane 3(20μg), 3T3(lane 4(20μg), HeLa(lane 5(20μg) using RAP1GAP Antibody (HY-P82081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • RAP1GAP Antibody (YA1826) Product Image
    1/16
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • RAP1GAP Antibody (YA1826) Product Image
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg), C6(lane 3(20μg), 3T3(lane 4(20μg), HeLa(lane 5(20μg) using RAP1GAP Antibody (HY-P82081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • Experimental Validation Results for RAP1GAP Antibody (YA1826)
  • RAP1GAP Antibody (YA1826) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20μg), C6(lane 3(20μg), 3T3(lane 4(20μg), HeLa(lane 5(20μg) using RAP1GAP Antibody (HY-P82081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human gastric cancer tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • RAP1GAP Antibody (YA1826) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human pancreas tissue using RAP1GAP Antibody (YA1826). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82081, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • RAP1GAP Antibody (YA1826) Product Image WB
  • RAP1GAP Antibody (YA1826) Product Image IHC-P
  • RAP1GAP Antibody (YA1826) Product Image IHC-P
  • RAP1GAP Antibody (YA1826) Product Image IHC-P
  • RAP1GAP Antibody (YA1826) Product Image IHC-P
  • RAP1GAP Antibody (YA1826) Product Image mIHC
  • RAP1GAP Antibody (YA1826) Product Image mIHC
  • RAP1GAP Antibody (YA1826) Product Image mIHC
  • RAP1GAP Antibody (YA1826) Product Image mIHC