Cyclin D2/CCND2 Antibody (YA789)

Cat. No.: HY-P80634: Data Sheet COA
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Cyclin D2/CCND2 Antibody (YA789) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Cyclin D2/CCND2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cyclin D2/CCND2 Antibody (YA789) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Cyclin D2/CCND2 Antibody (YA789) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Cyclin D2/CCND2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 38 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P30279

Gene ID

894 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Cyclin D2.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Cyclin D2 (HY-P80634) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:18827403, PubMed:8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:18827403, PubMed:8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed:18827403, PubMed:8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:18827403, PubMed:8114739)

Subcellular Localization：Nucleus; Cytoplasm; Nucleus membrane; Cytoplasm

Isoforms & Post-Translational Modification：P30279 has 2 isomers: P30279-1: 33067 Da (predicted); P30279-2: 24461 Da (predicted).
Phosphorylation at Thr-280 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex;Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-280 phosphorylation (PubMed:33854235, PubMed:33854239). The DCX(AMBRA1) complex represents the major regulator of CCND2 stability during the G1/S transition (PubMed:33854235, PubMed:33854239). Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation (PubMed:22323446)

Subunit：Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex (PubMed:18827403, PubMed:8114739). The cyclin subunit imparts substrate specificity to the complex (PubMed:18827403, PubMed:8114739)

RRID

AB_3102639

Database

Entrez Gene: 894 Human

SwissProt: P30279 Human

OMIM: 615938 Human

Research Field

Cell Biology

Synonyms

KIAK0002.

Documentation

Select Batch:

Data Sheet (259 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Cyclin D2/CCND2 Antibody (YA789) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cyclin D2/CCND2 Antibody (YA789)

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