DENN Antibody (YA2858)(PBS only)

Cat. No.: HY-P83113A: Data Sheet User Guide for Antibodies
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DENN Antibody (YA2858) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DENN.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DENN Antibody (YA2858) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DENN.

Host

Rabbit

Molecular Weight

Predicted band size: 183 kDa;

Observed band size: 220 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8WXG6

Gene ID

8567 [NCBI]

Immunogen

A synthetic peptide of human DENN

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family (PubMed:18559336, PubMed:20937701). Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms (PubMed:18559336, PubMed:20937701). Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion (By similarity). Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity). Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity). Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity). Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PubMed:32761064). May link TNFRSF1A with MAP kinase activation (PubMed:9115275). May be involved in the regulation of TNFA-induced apoptosis (PubMed:11577081, PubMed:32761064)

Subcellular Localization：Cell membrane; Cytoplasm; Cell projection, axon

Expression：
Tissue_specificity:Expressed in testis, ovary, brain and heart (PubMed:8988362) . Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon (PubMed:9115275) . Expressed in liver (PubMed:9796103) ; Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid; Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid; Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid; Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid; Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid; Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid; Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid

Isoforms & Post-Translational Modification：Q8WXG6 has 8 isomers: Q8WXG6-1: 183303 Da (predicted); Q8WXG6-2: 178558 Da (predicted); Q8WXG6-3: 176455 Da (predicted); Q8WXG6-4: 173395 Da (predicted); Q8WXG6-5: 171392 Da (predicted); Q8WXG6-6: 164006 Da (predicted); Q8WXG6-7: 175917 Da (predicted); Q8WXG6-8: 171006 Da (predicted).

Subunit：Interacts (via death domain) with TNFRSF1A (via death domain) (PubMed:11577081, PubMed:9115275). Interacts with PIDD1 (PubMed:10825539). Interacts with YWHAZ (PubMed:16959763). Interacts (via death domain) with KIF1B; links the motor KIF1B to Rab3-carrying vesicles in anterograde synaptic vesicle transport (By similarity). Interacts with KIF1A (By similarity). Interacts (via uDENN domain) with RAB3A, RAB3B, RAB3C and RAB3D; the GTP-bound form of the Rab proteins is preferred for interaction (By similarity)

Research Field

Synonyms

DENN; IG20; RAB3GEP

Documentation

User Guide for Antibodies (1077 KB)