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CDCA5 Antibody (YA2609)

(Synonyms: CDCA5; Cell division cycle associated protein 5; Cell division cycle-associated protein 5; p35; Sororin)
Customer Review

Based on 1 Customer Validation

CDCA5 Antibody (YA2609) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDCA5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • CDCA5 Antibody (YA2609) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50-1:100

Product Details

Description

CDCA5 Antibody (YA2609) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDCA5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database

Entrez Gene: 113130 Human

SwissProt: Q96FF9 Human

Immunogen

A synthesized peptide derived from human CDCA5 aa200-252/252.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293T (lane 2, 15 μg), Hela (lane 3, 15 μg), and A549 (lane 4, 15 μg) using CDCA5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), A549 (lane 6), and Caco-2 (lane 7) using CDCA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    CDCA5 is a Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair

  • Subcellular Localization

    Nucleus; Chromosome; Cytoplasm

  • Subunit

    Interacts with the APC/C complex (By similarity). Interacts with the chromatin-bound cohesin complex; the interaction is indirect, occurs after DNA replication and requires acetylation of the cohesin component SMC3. Interacts (via the FGF motif) with PDS5A and PDS5B; the interaction is direct and prevents the interaction of PDS5A with WAPL

  • SwissProt ID

    Q96FF9

  • Gene ID
    113130 [NCBI]
  • Synonyms

    CDCA5; Cell division cycle associated protein 5; Cell division cycle-associated protein 5; p35; Sororin

  • Research Field

    Cell Biology

CDCA5 Antibody (YA2609) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293T (lane 2, 15 μg), Hela (lane 3, 15 μg), and A549 (lane 4, 15 μg) using CDCA5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), A549 (lane 6), and Caco-2 (lane 7) using CDCA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image
    1/16
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • CDCA5 Antibody (YA2609) Product Image
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293T (lane 2, 15 μg), Hela (lane 3, 15 μg), and A549 (lane 4, 15 μg) using CDCA5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), A549 (lane 6), and Caco-2 (lane 7) using CDCA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • Experimental Validation Results for CDCA5 Antibody (YA2609)
  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293T (lane 2, 15 μg), Hela (lane 3, 15 μg), and A549 (lane 4, 15 μg) using CDCA5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), A549 (lane 6), and Caco-2 (lane 7) using CDCA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CDCA5 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82864, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDCA5 Antibody (YA2609) Product Image WB
  • CDCA5 Antibody (YA2609) Product Image WB
  • CDCA5 Antibody (YA2609) Product Image IHC-P
  • CDCA5 Antibody (YA2609) Product Image IHC-P
  • CDCA5 Antibody (YA2609) Product Image IHC-P
  • CDCA5 Antibody (YA2609) Product Image IHC-P
  • CDCA5 Antibody (YA2609) Product Image IHC-P
  • CDCA5 Antibody (YA2609) Product Image IHC-P