DDX5 Antibody (YA3838)
(Synonyms: p68; HLR1; G17P1; HUMP68)
Based on 1 Customer Validation
DDX5 Antibody (YA3838) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to DDX5.
-
Host:
Mouse
-
Isotype:
IgG2a
-
Application:
WB, IHC-P, FC, ELISA
-
Reactivity :
Human, Mouse, Monkey
-
Formulation:
Supplied in PBS with 0.05% sodium azide
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
DDX5 Antibody (YA3838) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to DDX5.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman, Mouse, Monkey
-
Observed Molecular WeightObserved band size: 69 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 69 kDa
Purified recombinant fragment of human DDX5 (AA: 475-614) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS with 0.05% sodium azide
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
DDX5 is involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms
-
Subcellular Localization
Nucleus; Nucleus, nucleolus; Nucleus speckle; Cytoplasm
-
Isoforms & Post-Translational Modification
P17844 has 2 isomers: P17844-1: 69148 Da (predicted); P17844-2: 60563 Da (predicted).
Arg-502 is dimethylated, probably to asymmetric dimethylarginine;Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination;Polyubiquitinated, leading to proteasomal degradation;Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues -
Subunit
Identified in the spliceosome C complex (PubMed:11991638). Component of a ribonucleoprotein complex containing mRNAs and RNA-binding proteins including DDX5, HNRNPH2 and SRSF1 as well as splicing regulator ARVCF (PubMed:24644279).
-
SwissProt ID
-
Synonyms
p68; HLR1; G17P1; HUMP68
Documentation
Powered by Bioz
