DDX5 - DEAD-box helicase 5 Gene

Also Known as p68; HLR1; G17P1; HUMP68

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 1655

About DDX5

Cytogenetic location: 17q23.3 Genomic coordinates (GRCh38): 17:64,498,254-64,506,866 (from NCBI)

This gene has 41 transcripts (splice variants), 275 orthologues, 38 paralogues and is associated with 48 phenotypes. Ubiquitous expression in bone marrow (RPKM 190.1), lymph node (RPKM 152.4) and 25 other tissues.

Summary

This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of Other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in Cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]

DDX5 Products (4)

mRNA	Protein	Name
NM_001320595.2	NP_001307524.1	probable ATP-dependent RNA helicase DDX5 isoform a
NM_001320596.3	NP_001307525.1	probable ATP-dependent RNA helicase DDX5 isoform a
NM_001320597.2	NP_001307526.1	probable ATP-dependent RNA helicase DDX5 isoform b
NM_004396.5	NP_004387.1	probable ATP-dependent RNA helicase DDX5 isoform a

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables MH2 domain binding	IPI IPI: Inferred from physical interaction	18548003	GOA
enables R-SMAD binding	IPI IPI: Inferred from physical interaction	18548003	GOA
enables RNA helicase activity	IMP IMP: Inferred from mutant phenotype	21343338	GOA
enables SMAD binding	IPI IPI: Inferred from physical interaction	18548003	GOA
enables mRNA 3'-UTR binding	IDA IDA: Inferred from direct assay	23788676	GOA
enables nuclear androgen receptor binding	IDA IDA: Inferred from direct assay	18829551	GOA
enables pre-mRNA binding	IDA IDA: Inferred from direct assay	21343338	GOA
enables primary miRNA binding	IDA IDA: Inferred from direct assay	18548003	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	10837141	GOA
enables ribonucleoprotein complex binding	IDA IDA: Inferred from direct assay	23788676	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in BMP signaling pathway	IMP IMP: Inferred from mutant phenotype	18548003	GOA
involved in alternative mRNA splicing, via spliceosome	IMP IMP: Inferred from mutant phenotype	24275493	GOA
involved in androgen receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	24275493	GOA
involved in epithelial to mesenchymal transition	IMP IMP: Inferred from mutant phenotype	24910439	GOA
involved in estrogen receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	24275493	GOA
involved in intrinsic apoptotic signaling pathway by p53 class mediator	IMP IMP: Inferred from mutant phenotype	15660129	GOA
involved in mRNA transcription	IMP IMP: Inferred from mutant phenotype	23143267	GOA
involved in negative regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	15298701	GOA
involved in nuclear-transcribed mRNA catabolic process	IDA IDA: Inferred from direct assay	23788676	GOA
involved in positive regulation of DNA damage response, signal transduction by p53 class mediator	IMP IMP: Inferred from mutant phenotype	15660129	GOA
involved in primary miRNA processing	IMP IMP: Inferred from mutant phenotype	18548003	GOA
involved in regulation of alternative mRNA splicing, via spliceosome	IDA IDA: Inferred from direct assay	21343338	GOA
involved in regulation of alternative mRNA splicing, via spliceosome	IMP IMP: Inferred from mutant phenotype	23022728	GOA
involved in regulation of androgen receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	18829551	GOA
NOT involved in regulation of miRNA transcription	IMP IMP: Inferred from mutant phenotype	18548003	GOA
involved in regulation of transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	24275493	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of catalytic step 2 spliceosome	IDA IDA: Inferred from direct assay	11991638	GOA
located in nuclear speck	IDA IDA: Inferred from direct assay	24644279	GOA
located in nucleolus	IDA IDA: Inferred from direct assay	10837141	GOA
located in nucleus	IDA IDA: Inferred from direct assay	24910439	GOA
part of ribonucleoprotein complex	IDA IDA: Inferred from direct assay	18809582	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

DDX5 Protein Structure

DEAD

Helicase_C

P68HR

0
100
200
300
400
500
614 a.a.

Protein Preferred Names	Protein Names
probable ATP-dependent RNA helicase DDX5	ATP-dependent RNA helicase DDX5

DDX5 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	DDX5	P17844	SMAD1	Homo sapiens	Q15797	Anti Tag CoIP	18548003
Intra	DDX5	P17844	SMAD1	Homo sapiens	Q15797	Anti Bait CoIP	18548003
Intra	DDX5	P17844	SMAD1	Homo sapiens	Q15797	Pull Down	18548003
Intra	DDX5	P17844	MBD3	Homo sapiens	O95983	Anti Bait CoIP	20676135
Intra	DDX5	P17844	POLR2A	Homo sapiens	P24928	Pull Down	12527917
Intra	DDX5	P17844	POLR2A	Homo sapiens	P24928	Anti Tag CoIP	17011493
Cross	DDX5	P17844	Crebbp	Mus musculus	P45481	Anti Tag CoIP	12527917
Cross	DDX5	P17844	Crebbp	Mus musculus	P45481	Pull Down	12527917
Intra	DDX5	P17844	HDAC1	Homo sapiens	Q13547	GMS	15298701
Intra	DDX5	P17844	HDAC1	Homo sapiens	Q13547	Anti Tag CoIP	15298701
Intra	DDX5	P17844	NFAT5	Homo sapiens	O94916	Anti Bait CoIP	22266867
Intra	DDX5	P17844	NFAT5	Homo sapiens	O94916	Anti Tag CoIP	22266867
Intra	DDX5	P17844	SMAD3	Homo sapiens	P84022	Pull Down	18548003
Intra	DDX5	P17844	EXOSC10	Homo sapiens	Q01780	PLA	23143267
Intra	DDX5	P17844	FBL	Homo sapiens	P22087	Anti Bait CoIP	10837141
Intra	DDX5	P17844	FBL	Homo sapiens	P22087	Y2H	10837141
Intra	DDX5	P17844	TP53	Homo sapiens	P04637	Pull Down	15660129
Intra	DDX5	P17844	TARDBP	Homo sapiens	Q13148	Y2H Array	32814053
Intra	DDX5	P17844	TARDBP	Homo sapiens	Q13148	Y2H Pooling	32814053
Intra	DDX5	P17844	TARDBP	Homo sapiens	Q13148	Validated Y2H	32814053
Intra	DDX5	P17844	TP53	Homo sapiens	P04637-1	Pull Down	20818423
Intra	DDX5	P17844	TP53	Homo sapiens	P04637-7	Pull Down	20818423
Intra	DDX5	P17844	TP53	Homo sapiens	P04637-7	Anti Bait CoIP	20818423
Intra	DDX5	P17844	EP300	Homo sapiens	Q09472	Confocal	12527917
Intra	DDX5	P17844	EP300	Homo sapiens	Q09472	Anti Bait CoIP	12527917
Intra	DDX5	P17844	EP300	Homo sapiens	Q09472	Pull Down	12527917
Intra	DDX5	P17844	CHD3	Homo sapiens	Q12873	Anti Bait CoIP	20676135
Intra	DDX5	P17844	DROSHA	Homo sapiens	Q9NRR4	Anti Bait CoIP	18548003
Cross	DDX5	P17844	Runx2	Mus musculus	Q08775-3	Pull Down	17960593
Intra	DDX5	P17844	SMAD5	Homo sapiens	Q99717	Anti Bait CoIP	18548003
Intra	DDX5	P17844	SMAD5	Homo sapiens	Q99717	Pull Down	18548003
Intra	DDX5	P17844	SF1	Homo sapiens	Q15637	Y2H Fragment Pooling	26420826
Intra	DDX5	P17844	DDX17	Homo sapiens	Q92841	Y2H	22365833
Intra	DDX5	P17844	DDX17	Homo sapiens	Q92841	Anti Tag CoIP	35271311
Intra	DDX5	P17844	IPO11	Homo sapiens	Q9UI26	Anti Tag CoIP	35271311
Cross	DDX5	P17844	PB1	Influenza A virus	Q1K9H5	Y2H	21994455
Cross	DDX5	P17844	PB1	Influenza A virus	Q1K9H5	Pull Down	21994455
Cross	DDX5	P17844	P27958-PRO_0000037577	Hepatitis C virus	P27958-PRO_0000037577	Anti Tag CoIP	15113910
Cross	DDX5	P17844	P27958-PRO_0000037577	Hepatitis C virus	P27958-PRO_0000037577	Anti Tag CoIP	22640416
Cross	DDX5	P17844	P27958-PRO_0000037577	Hepatitis C virus	P27958-PRO_0000037577	Pull Down	22640416
Cross	DDX5	P17844	P29991-PRO_0000308465	Dengue virus type 2	P29991-PRO_0000308465	Y2H	21911577

Cross: Cross-species interaction Intra: Intraspecies interaction

DDX5 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P82921	DDX5 Antibody (YA2666)	WB, IHC-F, IHC-P, ICC/IF, IP	Human, Mouse, Rat
HY-P82921A	DDX5 Antibody (YA2666)(PBS only)	WB, IHC-F, IHC-P, ICC/IF, IP	Human, Mouse, Rat
HY-P84141	DDX5 Antibody (YA3838)	WB, IHC-P, FC, ELISA	Human, Mouse, Monkey
HY-P84141A	DDX5 Antibody (YA3838)(PBS only)	WB, IHC-P, FC, ELISA	Human, Mouse, Monkey
HY-P86445	DDX5 Antibody (YA6137)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Lung Cancer	Lung Carcinoma Non-Small Cell Lung Carcinoma Lung Cancer, Susceptibility To Lung Cancer, Protection Against Adenocarcinoma Of Lung, Somatic Adenocarcinoma Of Lung, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer Lung Neoplasm Carcinoma Of Lung Lung Non-Small Cell Carcinoma Non-Small Cell Lung Cancer Nsclc Lung Neoplasms Malignant Neoplasm Of Lung Alveolar Cell Carcinoma Nonsmall Cell Lung Cancer, Somatic Nonsmall Cell Lung Cancer, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer, Susceptibility To Lung Cancer, Somatic Lung Cancer, Resistance To Cancer Of Lung Cancer Of Bronchus Cancer Of The Lung Lung Malignancies Lung Malignant Tumors Malignant Lung Tumor Malignant Tumor Of Lung Pulmonary Cancer Pulmonary Carcinoma Pulmonary Neoplasms Respiratory Carcinoma LNCR Adenocarcinoma Of Lung Neoplasm Of Lung Cancer Lung Carcinoma Non-Small Cell Lung Carcinoma, Non-Small-Cell Lung Lung Cancers Lung Carcinomas Cancer, Lung Cancer, Lung, Non-Small Cell Primary Malignant Neoplasm Of Lung Bronchioloalveolar Adenocarcinoma

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Bioactive Molecules (2)

Cat. No.	Product Name	Effect	Purity	Phase
HY-168739	Topoisomerase I inhibitor 17		/	Phase 2
HY-12486	FL118		99.22%	Phase 1

Pre-clinical Phase

Bioactive Molecules (8)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-178226	PBX-7016	Inhibitor	/	Unkown
HY-160438B	PBX-7011 TFA	Inhibitor	98.60%	Unkown
HY-160438A	PBX-7011 mesylate	Inhibitor	/	Unkown
HY-160438	PBX-7011	Inhibitor	/	Unkown
HY-181018	Topo I/DDX5-IN-1		/	Unkown
HY-RS03655	DDX5 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS16886	Ddx5 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23328	Ddx5 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	DDX5	RGD	RGD:619906
Canis familiaris	DDX5	VGNC	VGNC:39862
Bos taurus	DDX5	VGNC	VGNC:27972
Mus musculus	DDX5	MGD	MGI:105037
Macaca mulatta	DDX5	VGNC	VGNC:71763
Felis catus	DDX5	VGNC	VGNC:97398
Others	DDX5	NCBI