POLR2A - RNA polymerase II subunit A Gene

Also Known as RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 5430

About POLR2A

Cytogenetic location: 17p13.1 Genomic coordinates (GRCh38): 17:7,484,366-7,514,616 (from NCBI)

This gene has 10 transcripts (splice variants), 1 gene allele, 197 orthologues, 2 paralogues and is associated with 2 phenotypes. Ubiquitous expression in testis (RPKM 59.5), skin (RPKM 31.0) and 25 other tissues.

Summary

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several Other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

POLR2A Products (1)

mRNA	Protein	Name
NM_000937.5	NP_000928.1	DNA-directed RNA polymerase II subunit RPB1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables DNA-directed 5'-3' RNA polymerase activity	IDA IDA: Inferred from direct assay	9852112	GOA
enables RNA-dependent RNA polymerase activity	IDA IDA: Inferred from direct assay	23395899	GOA
enables kinase binding	IPI IPI: Inferred from physical interaction	28076779	GOA
enables magnesium ion binding	IDA IDA: Inferred from direct assay	27193682	GOA
enables microfibril binding	EXP EXP: Inferred from Experiment	28945358	GOA
enables microfibril binding	IDA IDA: Inferred from direct assay	28945358	GOA
enables promoter-specific chromatin binding	IDA IDA: Inferred from direct assay	24441171	GOA
contributes to protein binding	IPI IPI: Inferred from physical interaction	16239144	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	10075709	GOA
enables ubiquitin protein ligase binding	IPI IPI: Inferred from physical interaction	17996703	GOA
enables zinc ion binding	IDA IDA: Inferred from direct assay	27193682	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA-templated transcription termination	IMP IMP: Inferred from mutant phenotype	26700805	GOA
involved in positive regulation of RNA splicing	IDA IDA: Inferred from direct assay	21536736	GOA
involved in transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	9852112	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of Prp19 complex	IDA IDA: Inferred from direct assay	21536736	GOA
part of RNA polymerase II, core complex	IDA IDA: Inferred from direct assay	9852112	GOA
NOT located in nucleolus	IDA IDA: Inferred from direct assay	16109376	GOA
located in nucleus	IDA IDA: Inferred from direct assay	9852112	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

POLR2A Protein Structure

RNA_pol_Rpb1_1

RNA_pol_Rpb1_2

RNA_pol_Rpb1_3

RNA_pol_Rpb1_4

RNA_pol_Rpb1_5

RNA_pol_Rpb1_6

RNA_pol_Rpb1_7

RNA_pol_Rpb1_R

0
400
800
1200
1600
1970 a.a.

Protein Preferred Names	Protein Names
DNA-directed RNA polymerase II subunit RPB1	DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit

POLR2A Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	POLR2A	P24928	RPRD1A	Homo sapiens	Q96P16	Anti Bait CoIP	24997600
Cross	POLR2A	P24928	ORF6	Equid herpesvirus 1	L8B1Q7	Pull Down	24418534
Cross	POLR2A	P24928	ORF6	Equid herpesvirus 1	L8B1Q7	Anti Bait CoIP	24418534
Intra	POLR2A	P24928	SETX	Homo sapiens	Q7Z333	Anti Bait CoIP	26700805
Intra	POLR2A	P24928	SETX	Homo sapiens	Q7Z333	Anti Tag CoIP	26700805
Intra	POLR2A	P24928	DICER1	Homo sapiens	Q9UPY3-1	Anti Bait CoIP	24814348
Intra	POLR2A	P24928	RECQL5	Homo sapiens	O94762-1	Pull Down	18562274
Intra	POLR2A	P24928	RECQL5	Homo sapiens	O94762-1	Anti Tag CoIP	18562274
Intra	POLR2A	P24928	RECQL5	Homo sapiens	O94762-1	Far-WB	18562274
Intra	POLR2A	P24928	THAP3	Homo sapiens	Q8WTV1	Validated Y2H	32814053
Intra	POLR2A	P24928	THAP3	Homo sapiens	Q8WTV1	Y2H Array	32814053
Intra	POLR2A	P24928	THAP3	Homo sapiens	Q8WTV1	Y2H Pooling	32814053
Intra	POLR2A	P24928	MIER1	Homo sapiens	Q8N108-16	Validated Y2H	32814053
Intra	POLR2A	P24928	MIER1	Homo sapiens	Q8N108-16	Y2H Array	32814053
Intra	POLR2A	P24928	MIER1	Homo sapiens	Q8N108-16	Y2H Pooling	32814053
Intra	POLR2A	P24928	PAF1	Homo sapiens	Q8N7H5	Anti Bait CoIP	16024656
Intra	POLR2A	P24928	PAF1	Homo sapiens	Q8N7H5	Anti Bait CoIP	15923622
Intra	POLR2A	P24928	TCEA1	Homo sapiens	P23193	Pull Down	23748380
Intra	POLR2A	P24928	XAB2	Homo sapiens	Q9HCS7	CoIP	10944529
Intra	POLR2A	P24928	PRMT5	Homo sapiens	O14744	Anti Bait CoIP	26700805
Intra	POLR2A	P24928	DDX5	Homo sapiens	P17844	Anti Bait CoIP	12527917
Intra	POLR2A	P24928	TDRD3	Homo sapiens	Q9H7E2	Anti Tag CoIP	26700805
Intra	POLR2A	P24928	SMN1	Homo sapiens	Q16637	Anti Tag CoIP	26700805
Intra	POLR2A	P24928	SMN1	Homo sapiens	Q16637	Anti Bait CoIP	26700805
Intra	POLR2A	P24928	ASB6	Homo sapiens	Q9NWX5	Anti Tag CoIP	33961781
Intra	POLR2A	P24928	ASB6	Homo sapiens	Q9NWX5	Validated Y2H	32296183
Intra	POLR2A	P24928	SUPT5H	Homo sapiens	O00267	Anti Bait CoIP	24814348
Intra	POLR2A	P24928	SNF8	Homo sapiens	Q96H20	Pull Down	19819239
Intra	POLR2A	P24928	SNF8	Homo sapiens	Q96H20	Anti Tag CoIP	19819239
Intra	POLR2A	P24928	RPRD1B	Homo sapiens	Q9NQG5	Crosslink	22264791
Intra	POLR2A	P24928	CDC73	Homo sapiens	Q6P1J9	Anti Bait CoIP	15923622
Intra	POLR2A	P24928	CDC73	Homo sapiens	Q6P1J9	Anti Bait CoIP	16024656
Cross	POLR2A	P24928	PA	Influenza A virus	Q67020	BiFC	21994455

Cross: Cross-species interaction Intra: Intraspecies interaction

POLR2A Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80883	RNA Polymerase II Subunit B1 Antibody (YA090)	WB, IHC-F, IHC-P, ICC/IF, IP	Human
HY-P80883A	RNA Polymerase II Subunit B1 Antibody (YA090)(PBS only)	WB, IHC-F, IHC-P, ICC/IF, IP	Human
HY-P86338	RNA Polymerase II Subunit B1 Antibody (YA6030)	WB, IHC-P, ICC/IF, IP, ChIP, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Neurodevelopmental Disorder With Hypotonia And Variable Intellectual And Behavioral Abnormalities	NEDHIB
Skull Base Meningioma	Meningioma Of The Skull Base
Skull Base Cancer	Tumors Of Skull Base Skull Base Neoplasms
Fusariosis	Fusarium Infection
Herpes Simplex	Herpes Simplex Infections Herpesvirus Hominis Disease Herpes Simplex Disease Herpesviral Infection Due To Herpes Simplex Infections Due To Simplex Virus Herpes Nos
White Piedra	Tinea Blanca Trichosporosis Nodosa
Angiomatous Meningioma
Anterior Cranial Fossa Meningioma	Meningioma Of The Anterior Fossa
Cockayne Syndrome	Cockayne'S Syndrome Dwarfism-Retinal Atrophy-Deafness Syndrome Neill-Dingwall Syndrome Progeria-Like Syndrome Progeroid Nanism Cs
Olfactory Groove Meningioma	Meningioma Of The Olfactory Groove
Psammomatous Meningioma
Rhabdoid Meningioma	Papillary Meningioma Meningioma, Rhabdoid
Secretory Meningioma	Meningioma
Meningothelial Meningioma	Meningotheliomatous Meningioma
Cerebral Falx Meningioma	Falcine Meningioma Falx Cerebri Meningioma
Fibrous Meningioma	Fibroblastic Meningioma
Foramen Magnum Meningioma	Meningioma Of The Foramen Magnum
Hypotonia
Meningioma, Familial	Meningioma Familial Meningioma Meningioma, Familial, Susceptibility To Meningeal Neoplasm Meningeal Neoplasms Meningiomas Meningioma, Nf2-Related, Somatic Meningioma, Sis-Related Meningothelial Cell Tumor Neoplasm Of The Meninges Primary Meningeal Tumor Familial Multiple Meningioma MNGMA Meningioma, Benign, No Icd-O Subtype Intracranial Meningioma Meningothelial Cell Neoplasm Supratentorial Meningioma Primary Neoplasm Of Spinal Meninges Benign Intracranial Meningioma Benign Meningioma Meningeal Tumours Meningeal Sarcoma Of Unspecified Site Meningothelial Sarcoma Of Unspecified Site
Parasagittal Meningioma
Cavernous Sinus Meningioma	Meningioma Of The Cavernous Sinus
Cerebral Convexity Meningioma	Cerebral Hemispheric Convexity Meningioma
Clear Cell Meningioma
Transitional Meningioma	Transitional Meningioma
Myxoid Liposarcoma	Myxoid/Round Cell Liposarcoma Liposarcoma, Myxoid Mrcls Myxoliposarcoma MXLIPO Liposarcoma Myxoid Liposarcoma
Intraventricular Meningioma
Benign Meningioma	Meningioma, Benign
Ewing Sarcoma	Neuroepithelioma Ewing'S Tumor Primitive Neuroectodermal Tumor Ewings Sarcoma Ewing'S Sarcoma Peripheral Neuroepithelioma ES Ewings Sarcoma-Primitive Neuroectodermal Tumor Localized Peripheral Primitive Neuroectodermal Tumor Peripheral Primitive Neuroectodermal Tumor Ewing Tumor Sarcoma, Ewing'S Ewing Family Of Tumors Extraosseous Ewing Tumor Askin Tumor Ewing'S Family Localized Tumor Ewing'S Sarcoma/Peripheral Primitive Neuroectodermal Tumor Localized Ewing Sarcoma Localized Ewing'S Sarcoma Localized Ewing'S Sarcoma/Peripheral Primitive Neuroectodermal Tumor Localized Ewing'S Tumor Pnet Of Thoracopulmonary Region Tumor Of The Ewing Family Skeletal Ewing Sarcoma Osseous Ewing Sarcoma Ppnet Peripheral Pnet Extraskeletal Ewing Sarcoma Eoe Extraosseous Ewing Sarcoma Extraskeletal Ewing Tumor Esft Ewing Sarcoma Family Of Tumors Pne Pnet Pnet Of The Chest Wall Sarcoma, Ewing Neuroectodermal Tumors, Primitive, Peripheral Neuroectodermal Tumor, Primitive Disorder Of Eye Askin'S Tumor Extraosseous Ewings Sarcoma-Primitive Neuroepithelial Tumor Neuroepithelioma, Peripheral
Intracranial Meningioma	Meningioma
Cockayne Syndrome B	Cockayne Syndrome Type 2 Cockayne Syndrome, Type B Cockayne Syndrome Type Ii CSB Cockayne Syndrome 2 Cockayne Syndrome Type B Ckn2 Cockayne Syndrome, Type Ii

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (4)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-135646	Eleutheroside B1	Inhibitor	99.00%	Unkown
HY-RS10842	POLR2A Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS19802	Polr2a Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS26298	Polr2a Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Bos taurus	POLR2A	VGNC	VGNC:33135
Mus musculus	POLR2A	MGD	MGI:98086
Macaca mulatta	POLR2A	VGNC	VGNC:99209
Canis familiaris	POLR2A	VGNC	VGNC:44792
Rattus norvegicus	POLR2A	RGD	RGD:1587326
Felis catus	POLR2A	VGNC	VGNC:108139
Others	POLR2A	NCBI