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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Nicastrin Antibody (YA1397)

Nicastrin Antibody (YA1397)

Cat. No.: HY-P81652
COA
SDS
User Guide for Antibodies Technical Support

Nicastrin Antibody (YA1397) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicastrin.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Documentation

Description

Nicastrin Antibody (YA1397) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicastrin.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 78 kDa;
Observed band size: 110-120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Nicastrin
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Nicastrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335, PubMed:30598546, PubMed:30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels
Subcellular Localization：Membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Melanosome
Expression：
Tissue_specificity:Detected in brain (at protein level) (PubMed:10993067) . Widely expressed (PubMed:11396676)

Induction:Constitutively expressed in neural cells
Isoforms & Post-Translational Modification：Q92542 has 2 isomers: Q92542-1: 78411 Da (predicted); Q92542-2: 76744 Da (predicted).
N-glycosylated
Subunit：Component of the gamma-secretase complex (PubMed:10993067, PubMed:30598546, PubMed:30630874). The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2 (PubMed:12740439, PubMed:25043039, PubMed:25918421, PubMed:26280335, PubMed:26623517, PubMed:30598546, PubMed:30630874). Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP) (PubMed:10993067, PubMed:30630874). Interacts with PSEN1 and PSEN2 (PubMed:10993067)
RRID
Database

Entrez Gene: 23385 Human ; 59287 Mouse ; 289231 Rat

SwissProt: Q92542 Human ; P57716 Mouse ; Q8CGU6 Rat

OMIM: 142690 Human

Research Field

Neuroscience
Synonyms
APH2; Ncstn; NCT; Nicastrin
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Nicastrin Antibody (YA1397) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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