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EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)

(Synonyms: EPHX; EPOX; HYL1; MEH)
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Based on 1 Customer Validation

EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to EPHX1 (Epoxide Hydrolase 1).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:100

Product Details

Description

EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to EPHX1 (Epoxide Hydrolase 1).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 52.8 kDa
Immunogen

Human recombinant protein fragment corresponding to amino acids 21-230 of human EPHX1 produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

Product Properties

  • Appearance

    Liquid

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
    Western blot analysis of extracts from A549(lane 2, 20μg), A431(lane 3, 20μg), HeLa(lane 4, 20μg) and HepG2(lane 5, 20μg) using EPHX1 Antibody (HY-P88203). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
    Flow cytometric analysis of 1X106 HepG2 cells labeling EPHX1 Antibody (HY-P88203, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    EPHX1 is a Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol. Binds 20(S)-hydroxycholesterol (20(S)-OHC)[1][2].

  • Subcellular Localization

    Microsome membrane,Endoplasmic reticulum membrane

  • Expression


    Tissue_Specificity: Found in liver

  • Isoforms & Post-Translational Modification

    P07099: 455 amino acids, molecular weight 52949 Da.

  • SwissProt ID

    P07099

  • Synonyms

    EPHX; EPOX; HYL1; MEH

EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2, 20μg), A431(lane 3, 20μg), HeLa(lane 4, 20μg) and HepG2(lane 5, 20μg) using EPHX1 Antibody (HY-P88203). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HepG2 cells labeling EPHX1 Antibody (HY-P88203, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image
    1/16
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2, 20μg), A431(lane 3, 20μg), HeLa(lane 4, 20μg) and HepG2(lane 5, 20μg) using EPHX1 Antibody (HY-P88203). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HepG2 cells labeling EPHX1 Antibody (HY-P88203, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
  • Experimental Validation Results for EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887)
  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2, 20μg), A431(lane 3, 20μg), HeLa(lane 4, 20μg) and HepG2(lane 5, 20μg) using EPHX1 Antibody (HY-P88203). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HepG2 cells labeling EPHX1 Antibody (HY-P88203, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image WB
  • EPHX1 (Epoxide Hydrolase 1) Antibody (YA7887) Product Image FC