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CD68 Antibody (YA6052)

(Synonyms: CD68; Macrosialin; Gp110; CD68)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

CD68 Antibody (YA6052) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD68.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • CD68 Antibody (YA6052) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

CD68 Antibody (YA6052) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD68.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 90-110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

RRID

AB_3719130

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD68 Antibody (YA6052)
    Western blot analysis of extracts from RAW264.7 (lane2(20μg), J774A.1 (lane3(20μg), Mouse colon tissue (lane4(20μg) and Rat colon tissue (lane5(20μg) using CD68 Antibody (HY-P86360). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunohistochemical analysis of paraffin-embedded rat lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD68 Antibody (YA6052)
    Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CD68: This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Endosome membrane; Single-pass type I membrane protein; Lysosome membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts, and endothelial cells. It is expressed in many tumor cell lines, which may enable them to attach to selectins on vascular endothelium, thereby promoting their spread to secondary sites.

  • Isoforms & Post-Translational Modification

    CD68 has 3 isomers: P34810-1: 37408 Da (predicted); P34810-2: 31738 Da (predicted); P34810-3: 34654 Da (predicted).
    N- and O-glycosylated

  • SwissProt ID

    P34810

  • Gene ID
    968 [NCBI]
  • Synonyms

    CD68; Macrosialin; Gp110; CD68

CD68 Antibody (YA6052) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane2(20μg), J774A.1 (lane3(20μg), Mouse colon tissue (lane4(20μg) and Rat colon tissue (lane5(20μg) using CD68 Antibody (HY-P86360). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD68 Antibody (YA6052) Product Image
    1/16
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • CD68 Antibody (YA6052) Product Image
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane2(20μg), J774A.1 (lane3(20μg), Mouse colon tissue (lane4(20μg) and Rat colon tissue (lane5(20μg) using CD68 Antibody (HY-P86360). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD68 Antibody (YA6052)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • Experimental Validation Results for CD68 Antibody (YA6052)
  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7 (lane2(20μg), J774A.1 (lane3(20μg), Mouse colon tissue (lane4(20μg) and Rat colon tissue (lane5(20μg) using CD68 Antibody (HY-P86360). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD68 Antibody (YA6052) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling CD68 with CD68 Antibody (HY-P86360) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD68 Antibody (HY-P86360) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD68 Antibody (YA6052) Product Image WB
  • CD68 Antibody (YA6052) Product Image IHC-P
  • CD68 Antibody (YA6052) Product Image IHC-P
  • CD68 Antibody (YA6052) Product Image IHC-P
  • CD68 Antibody (YA6052) Product Image IHC-P
  • CD68 Antibody (YA6052) Product Image IHC-P
  • CD68 Antibody (YA6052) Product Image IHC-P
  • CD68 Antibody (YA6052) Product Image ICC
  • CD68 Antibody (YA6052) Product Image ICC