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CD66b Antibody (YA2071)

(Synonyms: CD66b; CD67; CEACAM8; CGM6; NCA95)
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Based on 1 Customer Validation

CD66b Antibody (YA2071) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD66b.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD66b Antibody (YA2071)
  • CD66b Antibody (YA2071) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

CD66b Antibody (YA2071) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD66b.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Species Reactivity Database

Entrez Gene: 1088 Human

SwissProt: P31997 Human

Immunogen

A synthesized peptide derived from human CD66b aa220-278/349.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD66b Antibody (YA2071)
    Western blot analysis was performed on extracts from A431 (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Hela (lane 3, 15 μg) using CD66b Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    CD66b cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Heterophilic interaction with CEACAM8 occurs in activated neutrophils[1][2][3].

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor; Cell surface

  • Expression


    Tissue_specificity:It is expressed in leukocytes and bone marrow of patients with chronic myeloid leukemia.

  • Subunit

    Monomer. Heterodimer with CEACAM6; heterodimerizes via its Ig-like V-type domain

  • SwissProt ID

    P31997

  • Gene ID
    1088 [NCBI]
  • Synonyms

    CD66b; CD67; CEACAM8; CGM6; NCA95

  • Research Field

    Immunology

CD66b Antibody (YA2071) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from A431 (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Hela (lane 3, 15 μg) using CD66b Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CD66b Antibody (YA2071) Product Image
    1/16
  • Experimental Validation Results for CD66b Antibody (YA2071)
  • CD66b Antibody (YA2071) Product Image
  • Experimental Validation Results for CD66b Antibody (YA2071)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from A431 (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Hela (lane 3, 15 μg) using CD66b Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CD66b Antibody (YA2071)
  • CD66b Antibody (YA2071) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from A431 (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Hela (lane 3, 15 μg) using CD66b Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CD66b Antibody (YA2071) Product Image WB