CD66b Antibody (YA2071)

Cat. No.: HY-P82326: Data Sheet COA
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User Guide for Antibodies
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CD66b Antibody (YA2071) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD66b.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD66b Antibody (YA2071) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

CD66b Antibody (YA2071) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD66b.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 38 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P31997

Gene ID

1088 [NCBI]

Immunogen

A synthesized peptide derived from human CD66b aa220-278/349.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from A431 (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Hela (lane 3, 15 μg) using CD66b Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:11590190, PubMed:2022629, PubMed:8776764). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:11590190, PubMed:2022629, PubMed:8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764)

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Cell surface

Expression：
Tissue_specificity:It is expressed in leukocytes and bone marrow of patients with chronic myeloid leukemia.

Subunit：Monomer. Heterodimer with CEACAM6; heterodimerizes via its Ig-like V-type domain

Database

Entrez Gene: 1088 Human

SwissProt: P31997 Human

OMIM: 615747 Human

Research Field

Immunology

Synonyms

CD66b; CD67; CEACAM8; CGM6; NCA95

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

CD66b Antibody (YA2071) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD66b Antibody (YA2071)

CD66b Antibody (YA2071) Featured Recommendations:

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Background

Documentation

CD66b Antibody (YA2071) Related Classifications

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