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Kaptin Antibody (YA9585)

(Synonyms: KICSTOR complex protein kaptin, Actin-associated protein 2E4, KPTN)
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Based on 1 Customer Validation

Kaptin Antibody (YA9585) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Kaptin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IP, ELISA

  • Reactivity :

    human

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Kaptin Antibody (YA9585)
  • Kaptin Antibody (YA9585) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1000 1:50-500 1:50-500 1-2μg per 100-500μg Total protein 1:50-3000

Product Details

Description

Kaptin Antibody (YA9585) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Kaptin.

  • Host Mouse
  • Species Reactivity
    human
  • Calculated Molecular Weight Predicted band size: 48 kDa
Immunogen

A synthesized peptide derived from human Kaptin.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Kaptin Antibody (YA9585)
    Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4) and Ramos (lane 5) using Kaptin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Kaptin is an As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose

  • Subcellular Localization

    Lysosome membrane; Cell projection, lamellipodium; Cell projection, stereocilium

  • Isoforms & Post-Translational Modification

    Kaptin has 2 isoforms, Q9Y664-1: amino acid length is 436, molecular weight is 48080 Da (predicted); Q9Y664-2: amino acid length is 196, molecular weight is 21583 Da (predicted).

  • Subunit

    Part of the KICSTOR complex composed of KPTN, ITFG2, KICS2 and SZT2.

  • SwissProt ID

    Q9Y664

  • Gene ID
    11133 [NCBI]
  • Synonyms

    KICSTOR complex protein kaptin, Actin-associated protein 2E4, KPTN

Kaptin Antibody (YA9585) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4) and Ramos (lane 5) using Kaptin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Kaptin Antibody (YA9585) Product Image
    1/16
  • Experimental Validation Results for Kaptin Antibody (YA9585)
  • Kaptin Antibody (YA9585) Product Image
  • Experimental Validation Results for Kaptin Antibody (YA9585)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4) and Ramos (lane 5) using Kaptin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Kaptin Antibody (YA9585)
  • Kaptin Antibody (YA9585) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4) and Ramos (lane 5) using Kaptin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Kaptin Antibody (YA9585) Product Image WB