GAD65 Antibody (YA758)

Cat. No.: HY-P80682: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

GAD65 Antibody (YA758) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to GAD65.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

GAD65 Antibody (YA758) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

GAD65 Antibody (YA758) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to GAD65.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 65 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q05329

Gene ID

2572 [NCBI]

Immunogen

Synthetic peptide corresponding to Human GAD2.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), Rat cerebellum (lane 3, 15 μg), and Rat hippocampus (lane 4, 15 μg) using GAD65 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Catalyzes the production of GABA

Subcellular Localization：Cytoplasm, cytosol; Cytoplasmic vesicle; Presynaptic cell membrane; Lipid-anchor; Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side

Subunit：Homodimer

RRID

AB_3102952

Database

Entrez Gene: 2572 Human ; 24380 Rat

SwissProt: Q05329 Human ; Q05683 Rat

OMIM: 138275 Human

Research Field

Neuroscience

Synonyms

GAD65

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

GAD65 Antibody (YA758) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

GAD65 Antibody (YA758)

GAD65 Antibody (YA758) Featured Recommendations:

Product Detail

Verification Image

Background

Documentation

GAD65 Antibody (YA758) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report