NUR77 Antibody (YA1462)(PBS only)

Cat. No.: HY-P81717A: Data Sheet User Guide for Antibodies
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NUR77 Antibody (YA1462) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NUR77.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NUR77 Antibody (YA1462) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NUR77.

Host

Rabbit

Molecular Weight

Predicted band size: 64 kDa;

Observed band size: 64 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P22736

Gene ID

3164 [NCBI]

Immunogen

A synthetic peptide of human NUR77 aa16-29.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:18690216, PubMed:8121493, PubMed:9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed:18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed:22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity); In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7

Subcellular Localization：Nucleus; Cytoplasm, cytosol; Mitochondrion

Expression：
Tissue_specificity:Fetal muscle and human liver, brain and thyroid

Induction:Induced by cytosporone B (Csn-B) ; directly stimulates its own expression (PubMed:18690216) . Induced by corticotropin-releasing hormone (CRH) (PubMed:9315652) . Induced by growth-stimulating agents (PubMed:1651101)

Isoforms & Post-Translational Modification：P22736 has 3 isomers: P22736-1: 64463 Da (predicted); P22736-2: 65998 Da (predicted); P22736-3: 33754 Da (predicted).
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli;Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1

Subunit：Binds the NGFI-B response element (NBRE) as a monomer (PubMed:18690216). Binds the Nur response element (NurRE), consisting of two inverse NBRE-related octanucleotide repeats separated by 6 base-pairs, as a dimer (PubMed:18690216, PubMed:9315652).

Research Field

Tags & Cell Markers

Synonyms

NR4A1; GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3

Documentation

User Guide for Antibodies (1077 KB)