HP1 alpha Antibody (YA5128)

Cat. No.: HY-P85436: Fiche technique User Guide for Antibodies
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HP1 alpha Antibody (YA5128) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to HP1 alpha.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description
Références

Description

HP1 alpha Antibody (YA5128) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to HP1 alpha.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 22 kDa;

Species Reactivity

Human, Mouse, Rat, Bovine, Dog, Pig

SwissProt ID

P45973

Gene ID

23468 [NCBI]

Immunogen

Purified recombinant human HP1α protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) (PubMed:19783980). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR) (PubMed:19783980). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1 (PubMed:19783980, PubMed:20231385). Required for the formation of the inner centromere (PubMed:20231385)

Subcellular Localization：Nucleus; Chromosome; Chromosome, centromere

Subunit：Homodimer (PubMed:20819937). Interacts with histone H3 methylated at 'Lys-9' (PubMed:11242053, PubMed:21047797).

Synonyms

CBX5; HP1A; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha

Documentation

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/mda-mb-468-cell.html [Content Brief]