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CEACAM6 Antibody (YA3351)

(Synonyms: CD66c; CEACAM6; CEAL; NCA; Non specific cross reacting antigen; Normal cross reacting antigen)
Customer Review

Based on 1 Customer Validation

CEACAM6 Antibody (YA3351) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEACAM6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL ICC IHC-P
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • CEACAM6 Antibody (YA3351) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50

Product Details

Description

CEACAM6 Antibody (YA3351) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEACAM6.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 40-100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database

Entrez Gene: 4680 Human ;

SwissProt: P40199 Human

Immunogen

A synthesized peptide derived from human CEACAM6 aa200-254/344.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105035

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CEACAM6 cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells. Plays a role in cell migration and cell adhesion to endothelial cells[1][2][3][4][5][6][7].

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor; Apical cell membrane; Cell surface

  • Expression


    Tissue_specificity:Expressed in neutrophils (PubMed:1378450) . Expressed in colonic columnar epithelial cells and goblet cells (PubMed:10436421) . Expressed in various tumor cell lines (protein level) (PubMed:16204051) .

  • Subunit

    Homodimer; homodimerizes via its Ig-like V-type domain. Heterodimer with CEACAM8; heterodimerizes via its Ig-like V-type domain

  • SwissProt ID

    P40199

  • Gene ID
    4680 [NCBI]
  • Synonyms

    CD66c; CEACAM6; CEAL; NCA; Non specific cross reacting antigen; Normal cross reacting antigen

  • Research Field

    Tags & Cell Markers

CEACAM6 Antibody (YA3351) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CEACAM6 Antibody (YA3351) Product Image
    1/16
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • CEACAM6 Antibody (YA3351) Product Image
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • Experimental Validation Results for CEACAM6 Antibody (YA3351)
  • CEACAM6 Antibody (YA3351) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CEACAM6 Antibody (YA3351) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling CEACAM6 with CEACAM6 Antibody(HY-P83606) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEACAM6 Antibody(HY-P83606) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CEACAM6 Antibody (YA3351) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CEACAM6 Antibody (YA3351) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using CEACAM6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CEACAM6 Antibody (YA3351) Product Image ICC
  • CEACAM6 Antibody (YA3351) Product Image ICC
  • CEACAM6 Antibody (YA3351) Product Image IHC-P
  • CEACAM6 Antibody (YA3351) Product Image IHC-P