Phospho-HER2 / ErbB2 (Tyr1139) Antibody (YA9919)
(Synonyms: ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340)
Based on 1 Customer Validation
Phospho-HER2 / ErbB2 (Tyr1139) Antibody (YA9919) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-HER2 / ErbB2 (Tyr1139).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:5000 | 1:10000 |
Product Details
Phospho-HER2 / ErbB2 (Tyr1139) Antibody (YA9919) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-HER2 / ErbB2 (Tyr1139).
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 180 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 138 kDa
Synthetic phosphopeptide corresponding to residues surrounding Tyr1139 of Human HER2.
Endogenous
affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HER2 / ErbB2 is a Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization; In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell projection, ruffle membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Early endosome; Cytoplasm, perinuclear region; Nucleus; Cytoplasm; Nucleus; Cytoplasm; Nucleus
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Expression
Tissue_specificity:This gene is expressed in a variety of tumor tissues, including primary breast tumors as well as tumors of the small intestine, esophagus, kidneys, and oral cavity. -
Isoforms & Post-Translational Modification
P04626 has 6 isomers: P04626-1: 137910 Da (predicted); P04626-2: 70917 Da (predicted); P04626-3: 62568 Da (predicted); P04626-4: 136501 Da (predicted); P04626-5: 134856 Da (predicted); P04626-6: 97382 Da (predicted).
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172, PubMed:33497358). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172) -
Subunit
Homodimer (PubMed:21454582). Heterodimer with EGFR, ERBB3 and ERBB4 (PubMed:10358079, PubMed:15093539, PubMed:16978839, PubMed:21190959).
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SwissProt ID
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Synonyms
ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340
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