Copy Link Print Information

Apolipoprotein E Antibody (YA6477)

(Synonyms: AD2 antibody; Apo-E antibody; APOE antibody; APOE_HUMAN antibody; APOEA antibody; Apolipoprotein E antibody; Apolipoprotein E3 antibody; ApolipoproteinE antibody; Apoprotein antibody; LDLCQ5 antibody; AD2 antibody; Apo-E antibody; APOE antibody; APOE_HUMAN antibody; APOEA antibody; Apolipoprotein E antibody; Apolipoprotein E3 antibody; ApolipoproteinE antibody; Apoprotein antibody; LDLCQ5 antibody; LPG antibody; )
Customer Review

Based on 1 Customer Validation

Apolipoprotein E Antibody (YA6477) is a Rat -derived and non-conjugated IgG1 monoclonal antibody, targeting to Apolipoprotein E.

For research use only. We do not sell to patients.
  • Host:

    Rat

  • Isotype:

    IgG1

  • Application:

    IHC-F, IHC-P, WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Apolipoprotein E Antibody (YA6477) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
Dilution Ratio 1:500 1:2000 1:5000

Product Details

Description

Apolipoprotein E Antibody (YA6477) is a Rat -derived and non-conjugated IgG1 monoclonal antibody, targeting to Apolipoprotein E.

  • Host Rat
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Apolipoprotein E is an APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE also has a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells[1].

  • Subcellular Localization

    Secreted,Secreted, extracellular space,Secreted, extracellular space, extracellular matrix,Extracellular vesicle,Endosome, multivesicular body

  • Isoforms & Post-Translational Modification

    P08226: 311 amino acids, molecular weight 35867 Da.
    N- and O-glycosylated丨Phosphorylated on Tyr-628 and Tyr-644 by SRC; these phosphorylations promote endothelial cell adhesion and migration

  • Subunit

    Homotetramer

  • SwissProt ID

    P08226

  • Gene ID
    11816 [NCBI]
  • Synonyms

    AD2 antibody; Apo-E antibody; APOE antibody; APOE_HUMAN antibody; APOEA antibody; Apolipoprotein E antibody; Apolipoprotein E3 antibody; ApolipoproteinE antibody; Apoprotein antibody; LDLCQ5 antibody; AD2 antibody; Apo-E antibody; APOE antibody; APOE_HUMAN antibody; APOEA antibody; Apolipoprotein E antibody; Apolipoprotein E3 antibody; ApolipoproteinE antibody; Apoprotein antibody; LDLCQ5 antibody; LPG antibody;

Apolipoprotein E Antibody (YA6477) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image
    1/16
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Apolipoprotein E Antibody (YA6477) Product Image
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Experimental Validation Results for Apolipoprotein E Antibody (YA6477)
  • Apolipoprotein E Antibody (YA6477) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Apolipoprotein E Antibody (HY-P86784, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Apolipoprotein E Antibody (YA6477) Product Image IHC-P
  • Apolipoprotein E Antibody (YA6477) Product Image IHC-P
  • Apolipoprotein E Antibody (YA6477) Product Image IHC-P
  • Apolipoprotein E Antibody (YA6477) Product Image IHC-P
  • Apolipoprotein E Antibody (YA6477) Product Image IHC-P
  • Apolipoprotein E Antibody (YA6477) Product Image IHC-P