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SERPING1 Antibody (YA1980)

(Synonyms: SERPING1; C1IN; C1NH; Plasma protease C1 inhibitor; C1 Inh; C1Inh; C1 esterase inhibitor; C1-inhibiting factor; Serpin G1)
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Based on 1 Customer Validation

SERPING1 Antibody (YA1980) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SERPING1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
  • SERPING1 Antibody (YA1980) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50

Product Details

Description

SERPING1 Antibody (YA1980) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SERPING1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human SERPING1 aa150-230.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SERPING1 Antibody (YA1980)
    Western blot analysis was performed on extracts from Mouse serum (lane 1, 15 μg), and Rat serum (lane 2, 15 μg) using SERPING1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
    Western blot analysis of extracts from A549 (lane 2(20μg), HepG2 (lane 3(20μg), HL-60 (lane 4(20μg) and Hela (lane 5(20μg) using SERPING1 Antibody (HY-P82235) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SERPING1 serine protease inhibitor, which acrs as a regulator of the classical complement pathway. Forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May also regulate blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein[1][2][3][4][5].

  • Subcellular Localization

    Secreted

  • Isoforms & Post-Translational Modification

    P05155 has 3 isomers: P05155-1: 55154 Da (predicted); P05155-2: 49757 Da (predicted); P05155-3: 55769 Da (predicted).
    Highly glycosylated (49%) with N- and O-glycosylation. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan heterogeneity at Asn-25: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor);Cleaved by C1S in vitro;(Microbial infection) Can be proteolytically cleaved by E.coli stcE

  • Subunit

    Interacts with MASP1

  • SwissProt ID

    P05155

  • Gene ID
    710 [NCBI]
  • Synonyms

    SERPING1; C1IN; C1NH; Plasma protease C1 inhibitor; C1 Inh; C1Inh; C1 esterase inhibitor; C1-inhibiting factor; Serpin G1

  • Research Field

    Cardiovascular

SERPING1 Antibody (YA1980) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Mouse serum (lane 1, 15 μg), and Rat serum (lane 2, 15 μg) using SERPING1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HepG2 (lane 3(20μg), HL-60 (lane 4(20μg) and Hela (lane 5(20μg) using SERPING1 Antibody (HY-P82235) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SERPING1 Antibody (YA1980) Product Image
    1/16
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
  • SERPING1 Antibody (YA1980) Product Image
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Mouse serum (lane 1, 15 μg), and Rat serum (lane 2, 15 μg) using SERPING1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HepG2 (lane 3(20μg), HL-60 (lane 4(20μg) and Hela (lane 5(20μg) using SERPING1 Antibody (HY-P82235) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
  • Experimental Validation Results for SERPING1 Antibody (YA1980)
  • SERPING1 Antibody (YA1980) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Mouse serum (lane 1, 15 μg), and Rat serum (lane 2, 15 μg) using SERPING1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • SERPING1 Antibody (YA1980) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg), HepG2 (lane 3(20μg), HL-60 (lane 4(20μg) and Hela (lane 5(20μg) using SERPING1 Antibody (HY-P82235) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SERPING1 Antibody (YA1980) Product Image WB
  • SERPING1 Antibody (YA1980) Product Image WB