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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Factor H Antibody (YA3361)

Factor H Antibody (YA3361)

Cat. No.: HY-P83616
COA
SDS
User Guide for Antibodies Technical Support

Factor H Antibody (YA3361) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Factor H.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Documentation

Description

Factor H Antibody (YA3361) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Factor H.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 139 kDa;
Observed band size: 180 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Factor H aa150-200/1231.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on protein extracts (30 μg) from A549 (lane 1) using Factor H antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background
Function：Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed:21285368, PubMed:21317894, PubMed:25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed:19503104, PubMed:21317894, PubMed:26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed:18252712, PubMed:23332154, PubMed:28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed:20008295, PubMed:9558116); (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination
Subcellular Localization：Secreted
Expression：
Tissue_specificity:Expressed in the retinal pigment epithelium (at protein level) (PubMed:25136834) . CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including monocytes, fibroblasts, or endothelial cells (PubMed:2139673, PubMed:25136834, PubMed:2968404, PubMed:6444659)
Isoforms & Post-Translational Modification：P08603 has 2 isomers: P08603-1: 139096 Da (predicted); P08603-2: 51034 Da (predicted).
Sulfated on tyrosine residues;According to a report, Asn-217 is not glycosylated (PubMed:17591618). Another study observed glycosylation at this position (PubMed:19139490)
Subunit：Homodimer (PubMed:18005991, PubMed:19505476). Also forms homooligomers (PubMed:19505476). Interacts with complement protein C3b; this interaction inhibits complement activation (PubMed:16601698, PubMed:19503104, PubMed:20378178, PubMed:21285368, PubMed:28671664). Interacts with complement protein C3d (PubMed:20378178, PubMed:21285368, PubMed:29190743). Interacts with CR3/ITGAM; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance (PubMed:20008295, PubMed:9558116). Interacts with complement factor I (PubMed:28671664)
Database

Entrez Gene: 3075 Human

SwissProt: P08603 Human

OMIM: 126700 Human

Research Field

Immunology
Synonyms
AHUS1; AMBP1; ARMD4; ARMS1; beta1H; CFH; CFHL3; complement factor H; isoform b; Factor H; factor H like 1; FHL1; HF1; HF2; HUS
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Factor H Antibody (YA3361) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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