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VEGFA Antibody (YA013)

(Synonyms: VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF)
6 Cited Publications
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Based on 6 publication(s) in Google Scholar

VEGFA Antibody (YA013) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VEGFA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VEGFA Antibody (YA013)
  • VEGFA Antibody (YA013) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

VEGFA Antibody (YA013) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VEGFA.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25-50 kDa; Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human VEGFA aa39-52 and aa139-154.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102759

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VEGFA Antibody (YA013)
    Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), HUVEC (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), A549 (lane 4, 20 μg), C6 (lane 5, 20 μg), and RAW264.7 (lane 6, 20 μg) using VEGFA Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity); Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437); Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth

  • Subcellular Localization

    Cytoplasm; Nucleus; Secreted; Endoplasmic reticulum; Golgi apparatus; Secreted, extracellular space, extracellular matrix; Secreted; Secreted; Secreted

  • Expression


    Tissue_specificity:Higher expression in pituitary tumors than in the pituitary gland; widespread expression; widespread expression; widespread expression; not widely expressed; not widely expressed

    Induction:By hypoxia. Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoglycemia and oncogenic mutations

  • Isoforms & Post-Translational Modification

    P15692 has 17 isomers: P15692-13: 43597 Da (predicted); P15692-1: 27042 Da (predicted); P15692-2: 25173 Da (predicted); P15692-3: 24422 Da (predicted); P15692-4: 22314 Da (predicted); P15692-5: 20218 Da (predicted); P15692-6: 20064 Da (predicted); P15692-8: 22259 Da (predicted); P15692-9: 17219 Da (predicted); P15692-10: 15981 Da (predicted); P15692-11: 40738 Da (predicted); P15692-12: 35643 Da (predicted); P15692-14: 45467 Da (predicted); P15692-15: 40683 Da (predicted); P15692-16: 42846 Da (predicted); P15692-17: 38642 Da (predicted); P15692-18: 34406 Da (predicted).
    Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form

  • Subunit

    Homodimer; disulfide-linked (By similarity). Interacts with NRP1 (PubMed:26503042). Interacts with isoform 2 of BSG (PubMed:25825981)

  • SwissProt ID

    P15692

  • Gene ID
    7422 [NCBI]
  • Synonyms

    VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF

  • Research Field

    Cardiovascular

VEGFA Antibody (YA013) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), HUVEC (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), A549 (lane 4, 20 μg), C6 (lane 5, 20 μg), and RAW264.7 (lane 6, 20 μg) using VEGFA Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • VEGFA Antibody (YA013) Product Image
    1/16
  • Experimental Validation Results for VEGFA Antibody (YA013)
  • VEGFA Antibody (YA013) Product Image
  • Experimental Validation Results for VEGFA Antibody (YA013)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), HUVEC (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), A549 (lane 4, 20 μg), C6 (lane 5, 20 μg), and RAW264.7 (lane 6, 20 μg) using VEGFA Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for VEGFA Antibody (YA013)
  • VEGFA Antibody (YA013) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), HUVEC (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), A549 (lane 4, 20 μg), C6 (lane 5, 20 μg), and RAW264.7 (lane 6, 20 μg) using VEGFA Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • VEGFA Antibody (YA013) Product Image WB