VEGFA Antibody (YA013)

Cat. No.: HY-P80929: Data Sheet COA
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VEGFA Antibody (YA013) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VEGFA.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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VEGFA Antibody (YA013) Featured Recommendations:

5 Publications Citing Use of MCE VEGFA Antibody (YA013)

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

VEGFA Antibody (YA013) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VEGFA.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 27 kDa;

Observed band size: 25-50 kDa;

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P15692

Gene ID

7422 [NCBI]

Immunogen

Synthetic peptide corresponding to Human VEGFA aa39-52 and aa139-154.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), HUVEC (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), A549 (lane 4, 20 μg), C6 (lane 5, 20 μg), and RAW264.7 (lane 6, 20 μg) using VEGFA Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity); Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437); Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth

Subcellular Localization：Cytoplasm; Nucleus; Secreted; Endoplasmic reticulum; Golgi apparatus; Secreted, extracellular space, extracellular matrix; Secreted; Secreted; Secreted

Expression：
Tissue_specificity:Higher expression in pituitary tumors than in the pituitary gland; widespread expression; widespread expression; widespread expression; not widely expressed; not widely expressed

Induction:By hypoxia. Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoglycemia and oncogenic mutations

Isoforms & Post-Translational Modification：P15692 has 17 isomers: P15692-13: 43597 Da (predicted); P15692-1: 27042 Da (predicted); P15692-2: 25173 Da (predicted); P15692-3: 24422 Da (predicted); P15692-4: 22314 Da (predicted); P15692-5: 20218 Da (predicted); P15692-6: 20064 Da (predicted); P15692-8: 22259 Da (predicted); P15692-9: 17219 Da (predicted); P15692-10: 15981 Da (predicted); P15692-11: 40738 Da (predicted); P15692-12: 35643 Da (predicted); P15692-14: 45467 Da (predicted); P15692-15: 40683 Da (predicted); P15692-16: 42846 Da (predicted); P15692-17: 38642 Da (predicted); P15692-18: 34406 Da (predicted).
Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form

Subunit：Homodimer; disulfide-linked (By similarity). Interacts with NRP1 (PubMed:26503042). Interacts with isoform 2 of BSG (PubMed:25825981)

RRID

AB_3102759

Database

Entrez Gene: 7422 Human ; 22339 Mouse ; 83785 Rat

SwissProt: P15692 Human ; Q96KJ0 Human ; Q00731 Mouse ; P16612 Rat

OMIM: 603933 Human

Research Field

Cardiovascular

Synonyms

VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

VEGFA Antibody (YA013) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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VEGFA Antibody (YA013)

VEGFA Antibody (YA013) Featured Recommendations:

5 Publications Citing Use of MCE VEGFA Antibody (YA013)

Product Detail

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Background

Documentation

VEGFA Antibody (YA013) Related Classifications

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