RBM25 Antibody
(Synonyms: RNPC7, RBM25, RNA-binding protein 25, Arg/Glu/Asp-rich protein of 120 kDa, Protein S164, RNA-binding motif protein 25, RNA-binding region-containing protein 7, RED120)RBM25 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RBM25.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
RBM25 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RBM25.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 17; 32; 33; 100 kDa
Purified recombinant protein of human RBM25.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RBM25 is a RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure
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Subcellular Localization
Nucleus speckle; Cytoplasm
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Isoforms & Post-Translational Modification
RBM25 has 4 isoforms, P49756-1: amino acid length is 843, molecular weight is 100185 Da (predicted); P49756-2: amino acid length is 294, molecular weight is 32822 Da (predicted); P49756-3: amino acid length is 300, molecular weight is 33510 Da (predicted); P49756-4: amino acid length is 164, molecular weight is 17953 Da (predicted).Sumoylated
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Subunit
Interacts with LUC7L3 and SRRM1.
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SwissProt ID
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Synonyms
RNPC7, RBM25, RNA-binding protein 25, Arg/Glu/Asp-rich protein of 120 kDa, Protein S164, RNA-binding motif protein 25, RNA-binding region-containing protein 7, RED120
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