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  5. Rabbit IgG Isotype Control

Rabbit IgG Isotype Control

Cat. No.: HY-P80879
COA
SDS
User Guide for Antibodies Technical Support

Rabbit IgG Antibody is a non-conjugated and Rabbit origined polyclonal antibody.It can be used as an isotype control.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

    Rabbit IgG Isotype Control purchased from MCE. Usage Cited in: Respir Res. 2025 Sep 29;26(1):275.  [Abstract]

    Binding of transcription factors CTCF, ZNF417, and ZNF184 at the SAE1 promoter (chr19: 47130617–47130965) was analyzed using ChIP-qPCR.

    Rabbit IgG Isotype Control purchased from MCE. Usage Cited in: Mol Med Rep. 2026 Jan;33(1):44.  [Abstract]

    Rabbit IgG Isotype Control (5 µg). Results of co-immunoprecipitation (Co-IP) of CDK1 and p53.
    • WB: Western Blot;
    • IHC-P: Immunohistochemistry-Paraffin;
    • IHC-F: Immunohistochemistry-Frozen;
    • ICC/IF: Immunocytochemistry/Immunofluorescence;
    • IF-Tissue: Immunofluorescence-Tissue;
    • mIHC: Multiplex Immunohistochemical;
    • IP: Immunoprecipitation;
    • ChIP: Chromatin Immunoprecipitation;
    • FC: Flow Cytometry;
    • ELISA: Enzyme Linked Immunosorbent Assay

    • Product Detail

    • Verification Image

    • Background

    • Documentation

    Description

    Rabbit IgG Antibody is a non-conjugated and Rabbit origined polyclonal antibody.It can be used as an isotype control.
    Host

    Rabbit
    Clonality

    Polyclonal
    Molecular Weight
    Predicted band size: 50 kDa;
    Observed band size: 50 kDa
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
    Species Reactivity
    Species independent
    SwissProt ID

    Gene ID
    Immunogen

    Synthetic peptide corresponding to Rabbit IgG.
    Application &
    Dilution Ratio
    Application Dilution Ratio
    WB
    WB: Western Blot
    		Assay-dependent
    IHC-P
    IHC-P: Immunohistochemistry-Paraffin
    		Assay-dependent
    IHC-F
    IHC-F: Immunohistochemistry-Frozen
    		Assay-dependent
    IF-Tissue
    IF-Tissue: Immunofluorescence-Tissue
    		Assay-dependent
    FC
    FC: Flow Cytometry
    		Assay-dependent
    ICC/IF
    ICC/IF: Immunocytochemistry/Immunofluorescence
    		Assay-dependent
    ELISA
    ELISA: Enzyme Linked Immunosorbent Assay
    		Assay-dependent
    Purity affinity purified Conjugation Non-conjugated
    Modification Unmodified Isotype IgG
    Appearance

    Solution
    Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
    Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    Shipping

    Shipping with blue ice.
    Verification Image
    WB

    • Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb (HY-P80879). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

    Background
    Function：Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the Target antibody.
    RRID
    Research Field

    Epigenetics and Nuclear Signaling
    Synonyms
    Rabbit IgG
    Documentation
    SDS (251 KB)

    COA (205 KB)

    User Guide for Antibodies (1077 KB)

    Rabbit IgG Isotype Control Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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