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Rabbit IgG Isotype Control

(Synonyms: Rabbit IgG)
4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

Rabbit IgG Antibody is a non-conjugated and Rabbit origined polyclonal antibody.It can be used as an isotype control.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP, FC, ELISA, IF-Tissue, mIHC

  • Reactivity :

    Species independent

  • Formulation:

    1.Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
    2.Supplied in 0.01M PBS, pH 7.4.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Rabbit IgG Isotype Control Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Rabbit IgG Isotype Control

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4 Publications Citing Use of MCE Rabbit IgG Isotype Control (2)
IP
  • IP
  • IP

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio Assay-dependent Assay-dependent Assay-dependent Assay-dependent Assay-dependent Assay-dependent Assay-dependent

Product Details

Description

Rabbit IgG Antibody is a non-conjugated and Rabbit origined polyclonal antibody.It can be used as an isotype control.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Synthetic peptide corresponding to Rabbit IgG.

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102988

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
    2.Supplied in 0.01M PBS, pH 7.4.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Room temperature in continental US; may vary elsewhere.

Verification Images

  • Experimental Validation Results for Rabbit IgG Isotype Control
    Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb (HY-P80879). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the Target antibody.

  • Synonyms

    Rabbit IgG

  • Research Field

    Epigenetics and Nuclear Signaling

Rabbit IgG Isotype Control Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb (HY-P80879). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    Rabbit IgG Isotype Control purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2026 Jan;33(1):44. [Abstract]

    Customer Validation
     Rabbit IgG Isotype Control (5 µg). Results of co-immunoprecipitation (Co-IP) of CDK1 and p53.

  • 1/16

    Rabbit IgG Isotype Control purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Sep 29;26(1):275. [Abstract]

    Customer Validation
     Binding of transcription factors CTCF, ZNF417, and ZNF184 at the SAE1 promoter (chr19: 47130617–47130965) was analyzed using ChIP-qPCR.

  • Rabbit IgG Isotype Control Product Image
    1/16
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Rabbit IgG Isotype Control Product Image
  • Experimental Validation Results for Rabbit IgG Isotype Control
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb (HY-P80879). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Rabbit IgG Isotype Control
  • Rabbit IgG Isotype Control Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb (HY-P80879). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Rabbit IgG Isotype Control Product Image WB