2. Inhibitory Antibodies
  3. Isotype Control Antibodies
  4. Mouse IgG1 kappa, Isotype Control

Mouse IgG1 kappa, Isotype Control 

Cat. No.: HY-P99977 Purity: 99.36%
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Mouse IgG1 kappa, Isotype Control is a negative control reagent/isotype control for mouse-derived IgG1κ antibodies. Mouse IgG1 kappa, Isotype Control, by maintaining the same immunoglobulin structure (including isotype, light chain, etc.) as the specific mouse IgG1κ subtype primary antibody, eliminates false positive signals caused by non-specific binding in immunological experiments, thus playing a core role in verifying the specificity of the experiment. Mouse IgG1 kappa, Isotype Control can be applied to immunolocalization experiments in the field of cell biology, such as immunofluorescence staining and immunohistochemistry, assisting in research on protein subcellular localization and cell structure analysis, ensuring the reliability of experimental results.

For research use only. We do not sell to patients.

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Customer Review

Based on 7 publication(s) in Google Scholar

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Mouse IgG1 kappa, Isotype Control Related Antibodies

Top Publications Citing Use of Products

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Jan;22(1):83-96.  [Abstract]

    Schematic diagram of the application of 4-OHT to the ears of HO (Ppargfl/fl;Krt5creERT2/+) mice for 5 consecutive days, along with intradermal injections of neutralizing antibodies to IFNAR (HY-P99137; Anti-Mouse IFNAR1 Antibody (MAR1-5A3)) or anti-mouse IgG1 isotype controls (HY-P99977; Mouse IgG1 kappa, Isotype Control) on days 3 and 7 (intradermally administered 40 μl of a 2 mg/ml solution). Quantification and qualification of urine protein.

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Jan;22(1):83-96.  [Abstract]

    Schematic diagram of the application of 4-OHT to the ears of HO (Ppargfl/fl;Krt5creERT2/+) mice for 5 consecutive days, along with intradermal injections of neutralizing antibodies to IFNAR (HY-P99137; Anti-Mouse IFNAR1 Antibody (MAR1-5A3)) or anti-mouse IgG1 isotype controls (HY-P99977; Mouse IgG1 kappa, Isotype Control) on days 3 and 7 (intradermally administered 40 μl of a 2 mg/ml solution). The levels of anti-double-stranded DNA antibodies and antinuclear antibodies in the peripheral blood of the mice on day 17.

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Leukemia. 2025 Aug 15.  [Abstract]

    Effect of cytokine signaling blockade on ALL cell survival in co-culture with stromal populations. ALL cells were co-cultured with early mesenchymal progenitors, adipogenic progenitors, or cultured alone. Cell survival was assessed after treatment with blocking antibodies against IL7, Osteopontin, or the CXCR4 inhibitor Plerixafor (1 nM; 7 days), Mouse IgG1 kappa, Isotype Control (1 ug/mL; 7 days).

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Leukemia. 2025 Aug 15.  [Abstract]

    Effect of cytokine signaling blockade on ALL cell survival in co-culture with stromal populations. ALL cells were co-cultured with early mesenchymal progenitors, adipogenic progenitors, or cultured alone. Effect of VCAM1 blockade on ALL cell viability in co-culture or monoculture. Effect of blocking integrin beta 1 (ITGB1) using a blocking antibody or the small peptidomimetic drug BIO-1211 (50 uM; 7 days), Mouse IgG1 kappa, Isotype Control (1 ug/mL; 7 days)on ALL cell viability in co-culture or monoculture.

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jul 23:S1074-7613(24)00351-0.  [Abstract]

    HUVECs were pretreated with IgG (Mouse IgG1 kappa, Isotype Control) or anti-Notch1 antibodies at 10 μg/mL for 2 h, then given control exosomes or stroke exosomes (15 μg/mL) for 24 h. mRNA expression of Notch1, VCAM1, P16, and P53 were assessed by quantitative real-time PCR. n = 6 per group.

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jul 23:S1074-7613(24)00351-0.  [Abstract]

    HUVECs were pretreated with IgG (Mouse IgG1 kappa, Isotype Control) or anti-Notch1 antibodies at 10 μg/mL for 2 h, then given control exosomes or stroke exosomes (15 μg/mL) for 24 h. Flow cytometry analysis of Notch1, VCAM1, P16, and P53 expression in HUVECs.

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jul 23:S1074-7613(24)00351-0.  [Abstract]

    HUVECs were pretreated with IgG (Mouse IgG1 kappa, Isotype Control) or anti-Notch1 mAb at 10 μg/mL for 2 h, then given control exosomes or stroke exosomes (15 μg/mL) for 24 h. THP-1 cells were labeled with fluorescence dye, then cell adhesion assay was performed. Representative images of adhesive cells.

    Mouse IgG1 kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jul 23:S1074-7613(24)00351-0.  [Abstract]

    Wild-type mice received intraperitoneal (i.p.) injections of IgG control (Mouse IgG1 kappa, Isotype Control ) or anti-VCAM1 mAb (5 mg/kg/week) following MCAO surgery. Flow cytometry analysis was conducted at day 14 after surgery. Counts of Ly6Chigh monocytes (left) and neutrophils (right) in aorta. n = 6 mice per group. Data were representative of three independent experiments.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Mouse IgG1 kappa, Isotype Control is a negative control reagent/isotype control for mouse-derived IgG1κ antibodies. Mouse IgG1 kappa, Isotype Control, by maintaining the same immunoglobulin structure (including isotype, light chain, etc.) as the specific mouse IgG1κ subtype primary antibody, eliminates false positive signals caused by non-specific binding in immunological experiments, thus playing a core role in verifying the specificity of the experiment. Mouse IgG1 kappa, Isotype Control can be applied to immunolocalization experiments in the field of cell biology, such as immunofluorescence staining and immunohistochemistry, assisting in research on protein subcellular localization and cell structure analysis, ensuring the reliability of experimental results[1][2].

    In Vitro

    Mouse IgG1 kappa Isotype Control is primarily used as a negative control in immunofluorescence staining experiments. When using mouse IgG1 subtype monoclonal antibodies for protein localization studies, it helps eliminate interference from non-specific antibody binding, allowing researchers to determine whether the staining signal of the target antibody is due to specific binding with the antigen, thus providing a basis for the specificity and reliability of the experimental results[1].
    It is especially suitable for experimental scenarios involving simultaneous immunolocalization with two different subtypes of mouse monoclonal antibodies (such as IgG1 and IgG1)[1].


    Applicable Features 1. No specific target, does not specifically bind to the target antigen in the experiment, only provides a background baseline for non-specific binding[1].

    2. Subtype-specific matching: It must match the target primary antibody (mouse IgG1 subtype) used in the experiment to accurately eliminate subtype-related non-specific binding interference[1].

    3. Does not possess biological activity such as agonism or inhibition; it only serves as an experimental control tool and does not affect the structure and function of the cells themselves[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Mouse IgG1 kappa, Isotype Control Related Antibodies
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    Molecular Weight

    145.06 kDa

    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Mouse IgG1 kappa, Isotype Control]
    Shipping

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation

    Purity: 99.36%

    SDS (251 KB)

    COA (232 KB) SDS-PAGE (118 KB) SEC-HPLC (107 KB)

    Inhibitory Antibodies User Guide (603 KB)
    References
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    Mouse IgG1 kappa, Isotype Control Related Classifications

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mouse IgG1 kappa, Isotype ControlOthersInhibitorinhibitorinhibit

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