Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) 

Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) is a mouse-derived Osteopontin/SPP1 IgG1 κ type antibody inhibitor. Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) blocks Angiotensin II (HY-13948)-induced DNA synthesis and collagen gel contraction in cardiac fibroblasts. Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) significantly inhibits tumor growth in CT26 or MC38 tumors mice models^[1][2][3].

Mouse IgG1 kappa

Mouse IgG1 kappa, Isotype Control

Human/Mouse/Rat

Osteopontin/SPP1

Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) (7.2 μg/mL or 72 ng/mL, 30 min) blocks Angiotensin II (HY-13948)-induced DNA synthesis in cardiac fibroblasts^[2].
Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) (7.2 μg/mL or 25 μg/mL, 30 min) blocks both Angiotensin II-induced and osteopontin-induced collagen gel contraction in cardiac fibroblasts^[2].
Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) (100 ng/mL, 48 h) significantly attenuates VSMC (vascular smooth muscle cells) migration induced by HMGB1 (high-mobility group box 1) in A10 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10) (500 μg/mouse, i.p., every other day for 10 days) significantly inhibits tumor growth in CT26 or MC38 tumors mice models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

20750  [NCBI]

P10923

in vivo OPN neutralization; in vitro OPN neutralization; Immunohistochemistry (paraffin); Western

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse/Rat/Human Osteopontin/SPP1 Antibody (MPIIIB10)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1X10⁶ A549 cells with Anti-Mouse/Rat/Human-SPP1 Antibody (MPIIIB10) (HY-P990117, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Kim EK, et al. Tumor-derived osteopontin suppresses antitumor immunity by promoting extramedullary myelopoiesis. Cancer Res. 2014 Nov 15;74(22):6705-16.  [Content Brief]

  [2]. Ashizawa N, et al. Osteopontin is produced by rat cardiac fibroblasts and mediates A(II)-induced DNA synthesis and collagen gel contraction. J Clin Invest. 1996 Nov 15;98(10):2218-27.  [Content Brief]

  [3]. Kim JY, et al. Echinochrome A inhibits HMGB1-induced vascular smooth muscle cell migration by suppressing osteopontin expression. Korean J Physiol Pharmacol. 2025 Jan 1;29(1):83-92.  [Content Brief]