Mouse IgG2a kappa, Isotype Control
Based on 2 publication(s) in Google Scholar
Mouse IgG2a kappa, Isotype Control is a negative control reagent/isotype control for mouse-derived IgG2aκ antibodies. Mouse IgG2a kappa, Isotype Control maintains the same immunoglobulin structure (including isotype, light chain, etc.) as the specific mouse IgG2aκ subtype primary antibody, thus eliminating false positive signals caused by non-specific adsorption in immunological experiments and playing a core role in verifying experimental specificity. Mouse IgG2a kappa, Isotype Control can be applied to immunolocalization experiments such as immunofluorescence staining and immunohistochemistry in the field of cell biology, assisting in protein subcellular localization, cell structure analysis, and other research to ensure the reliability of experimental results.
For research use only. We do not sell to patients.
- Purity: 99.54%
- Molecular Weight:143.58 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Mouse IgG2a kappa, Isotype Control
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Customer Validation & Images
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In Vivo Efficacy Study
Biological Activity
Mouse IgG2a kappa Isotype Control is primarily used as a negative control in immunofluorescence staining experiments. When using mouse IgG2a subtype monoclonal antibodies for protein localization studies, it helps eliminate interference from non-specific antibody binding, allowing researchers to determine whether the staining signal of the target antibody is due to specific binding with the antigen, thus providing a basis for the specificity and reliability of the experimental results[1].
It is especially suitable for experimental scenarios involving simultaneous immunolocalization with two different subtypes of mouse monoclonal antibodies (such as IgG1 and IgG2a)[1].
Applicable Features
1. No specific target, does not specifically bind to the target antigen in the experiment, and only provides a background baseline for non-specific binding[1].
2. Subtype-specific matching: It must match the target primary antibody (mouse IgG2a subtype) used in the experiment to accurately eliminate subtype-related non-specific binding interference[1].
3. It does not possess biological activity such as agonistic or inhibitory effects, and only serves as an experimental control tool, without affecting the structure and function of the cells themselves[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
Chemical Information
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Appearance Liquid
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Molecular Weight 143.58 kDa
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Color Colorless to light yellow
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SMILES
[Mouse IgG2a kappa, Isotype Control]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
Radiotherapy-resistant prostate cancer cells escape immune checkpoint blockade through the senescence-related ataxia telangiectasia and Rad3-related protein. [Abstract]2025 Mar;45(3):218-244. PMID: 39698847
Mouse IgG2a kappa, Isotype Control purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Mar;45(3):218-244. [Abstract]
Tumor volume and Survival analysis of control, control + anti‐Ctla‐4, RT‐resistant, and RT‐resistant + anti‐Ctla‐4 tumors in WT C57BL/6 mice (n = 6 for each group). The control group of mice received an equivalent concentration of the IgG2a isotype control antibody (200 µg; i.p.; days 7, 10, 13) (HY-P99978). The purple triangle indicates the anti‐Ctla‐4 injection. Abbreviations: RTres, radiotherapy resistant.
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Sci Rep
Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages. [Abstract]2024 Dec 30;14(1):31901. PMID: 39738316
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Mouse IgG2a kappa, Isotype Control Related Classifications
HY-P99978 Related Classifications
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