Mouse IgG2a kappa, Isotype Control 

Mouse IgG2a kappa, Isotype Control is a negative control reagent/isotype control for mouse-derived IgG2aκ antibodies. Mouse IgG2a kappa, Isotype Control maintains the same immunoglobulin structure (including isotype, light chain, etc.) as the specific mouse IgG2aκ subtype primary antibody, thus eliminating false positive signals caused by non-specific adsorption in immunological experiments and playing a core role in verifying experimental specificity. Mouse IgG2a kappa, Isotype Control can be applied to immunolocalization experiments such as immunofluorescence staining and immunohistochemistry in the field of cell biology, assisting in protein subcellular localization, cell structure analysis, and other research to ensure the reliability of experimental results^[1][2].

Mouse IgG2a kappa Isotype Control is primarily used as a negative control in immunofluorescence staining experiments. When using mouse IgG2a subtype monoclonal antibodies for protein localization studies, it helps eliminate interference from non-specific antibody binding, allowing researchers to determine whether the staining signal of the target antibody is due to specific binding with the antigen, thus providing a basis for the specificity and reliability of the experimental results^[1].
It is especially suitable for experimental scenarios involving simultaneous immunolocalization with two different subtypes of mouse monoclonal antibodies (such as IgG1 and IgG2a)^[1].


Applicable Features 1. No specific target, does not specifically bind to the target antigen in the experiment, and only provides a background baseline for non-specific binding^[1].

2. Subtype-specific matching: It must match the target primary antibody (mouse IgG2a subtype) used in the experiment to accurately eliminate subtype-related non-specific binding interference^[1].

3. It does not possess biological activity such as agonistic or inhibitory effects, and only serves as an experimental control tool, without affecting the structure and function of the cells themselves^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

143.58 kDa

Liquid

Colorless to light yellow

[Mouse IgG2a kappa, Isotype Control]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Donaldson JG, et al. Immunofluorescence Staining. Curr Protoc Cell Biol. 2015 Dec 1;69:4.3.1-4.3.7.  [Content Brief]

  [2]. Carter P, et al. Humanization of an anti-p185HER2 antibody for human cancer therapy. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4285-9.  [Content Brief]