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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies Modification Specific Antibodies
  5. Phospho-p27 KIP 1 (Ser10) Antibody (YA1494)

Phospho-p27 KIP 1 (Ser10) Antibody (YA1494)

Cat. No.: HY-P81749
COA
SDS
User Guide for Antibodies Technical Support

Phospho-p27 KIP 1 (Ser10) Antibody (YA1494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-p27 KIP 1 (Ser10).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈 가격 재고 수량
20 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 제품 설명

제품 설명

Phospho-p27 KIP 1 (Ser10) Antibody (YA1494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-p27 KIP 1 (Ser10).
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 22 kDa;
Observed band size: 27 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:5000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:20-1:50
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Solution
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Background
Function：Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry
Subcellular Localization：Nucleus; Cytoplasm; Endosome
Expression：
Tissue_specificity:It is expressed in the kidney (protein level) (PubMed: 15509543) . It is expressed in all tissues tested (PubMed: 8033212) . The highest content is found in skeletal muscle, and the lowest content is found in the liver and kidney (PubMed: 8033212) .

Induction:Maximal levels in quiescence cells and early G (1) . Levels decrease after mitogen stimulation as cells progress toward S-phase
Subunit：Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.
Database

Entrez Gene: 1027 Human ; 12576 Mouse ; 83571 Rat

SwissProt: P46527 Human ; P46414 Mouse

OMIM: 600778 Human

Research Field

Cell Biology
Synonyms
CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-p27 KIP 1 (Ser10) Antibody (YA1494) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Phospho-p27 KIP 1 (Ser10) Antibody (YA1494)
Cat. No.:
HY-P81749
수량:
MCE Japan Authorized Agent:

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English - EN (251 KB)

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