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CD3D Antibody (YA3639)

(Synonyms: T3D; IMD19; CD3-DELTA)
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Based on 1 Customer Validation

CD3D Antibody (YA3639) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD3D.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD3D Antibody (YA3639)
  • CD3D Antibody (YA3639) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

CD3D Antibody (YA3639) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD3D.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 19 kDa
Immunogen

Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3718799

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD3D Antibody (YA3639)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), HL-60 (lane 3(20μg), U937 (lane 4(20μg), THP-1 (lane 5(20μg) using CD3D Antibody（HY-P83942). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD3D is a Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells[1][2].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:CD3D is mainly found on T lymphocytes and binds to its TCR-CD3 chaperone protein. CD3D is also present in fetal NK cells.

  • Isoforms & Post-Translational Modification

    P04234 has 2 isomers: P04234-1: 18930 Da (predicted); P04234-2: 14484 Da (predicted).
    Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8

  • Subunit

    The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8 (PubMed:12215456, PubMed:1396954)

  • SwissProt ID

    P04234

  • Gene ID
    915 [NCBI]
  • Synonyms

    T3D; IMD19; CD3-DELTA

CD3D Antibody (YA3639) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HL-60 (lane 3(20μg), U937 (lane 4(20μg), THP-1 (lane 5(20μg) using CD3D Antibody（HY-P83942). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • CD3D Antibody (YA3639) Product Image
    1/16
  • Experimental Validation Results for CD3D Antibody (YA3639)
  • CD3D Antibody (YA3639) Product Image
  • Experimental Validation Results for CD3D Antibody (YA3639)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HL-60 (lane 3(20μg), U937 (lane 4(20μg), THP-1 (lane 5(20μg) using CD3D Antibody（HY-P83942). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD3D Antibody (YA3639)
  • CD3D Antibody (YA3639) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), HL-60 (lane 3(20μg), U937 (lane 4(20μg), THP-1 (lane 5(20μg) using CD3D Antibody（HY-P83942). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • CD3D Antibody (YA3639) Product Image WB