ADORA2A Antibody (YA3939)

Cat. No.: HY-P84242: Data Sheet COA
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ADORA2A Antibody (YA3939) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ADORA2A.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

ADORA2A Antibody (YA3939) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ADORA2A.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P29274

Gene ID

135 [NCBI]

Immunogen

Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB FC ICC

Western blot analysis of extracts from HEK293(lane 1(40μg) )
Western blot analysis of extracts from RAW264.7(lane 2(20μg) ,F9(lane 3(20μg) ,C2C12(lane 4(20μg)and 3T3(lane 5(20μg) using ADORA2A Antibody（HY-P84242）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from HEK293(lane 1(40μg) ),F9(lane 2(40μg) ),L1210(lane 3(40μg) ),C6(lane 4(40μg) ),NIH/3T3(lane 5(40μg) ),Raw264.7(lane 6(40μg) ),HepG2(lane 7(40μg) )and Hela(lane 8(40μg) ) using ADORA2A antibody(31299P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 Hela cells labeling ADORA2A Antibody(HY-P84242
Immunocytochemistry analysis of U251 cells labeling ADORA2A Antibody (YA3939, Mouse) with ADORA2A Antibody (YA3939, Mouse) (HY-P84242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADORA2A Antibody (YA3939, Mouse) ((HY-P84242) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunofluorescence analysis of Hela cells labeling ADORA2A antibody (HY-P84242) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

Function：Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Subunit：Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct (PubMed:16339847). May interact with DRD4 (PubMed:18832607). Interacts with NECAB2 (PubMed:17689978). Interacts (via cytoplasmic C-terminal domain) with GAS2L2; interaction enhances receptor-mediated adenylyl cyclase activity (By similarity)

RRID

AB_3718863

Synonyms

A2aR; RDC8; ADORA2

Documentation

Select Batch:

Data Sheet (258 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sw-620-cell.html [Content Brief]