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ADORA2A Antibody (YA3939)

(Synonyms: A2aR; RDC8; ADORA2)
Customer Review

Based on 1 Customer Validation

ADORA2A Antibody (YA3939) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ADORA2A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • ADORA2A Antibody (YA3939) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

ADORA2A Antibody (YA3939) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ADORA2A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Immunogen

Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3718863

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    Western blot analysis of extracts from HEK293(lane 1(40μg) )
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    Western blot analysis of extracts from RAW264.7(lane 2(20μg) ,F9(lane 3(20μg) ,C2C12(lane 4(20μg)and 3T3(lane 5(20μg) using ADORA2A Antibody（HY-P84242）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    Western blot analysis of extracts from HEK293(lane 1(40μg) ),F9(lane 2(40μg) ),L1210(lane 3(40μg) ),C6(lane 4(40μg) ),NIH/3T3(lane 5(40μg) ),Raw264.7(lane 6(40μg) ),HepG2(lane 7(40μg) )and Hela(lane 8(40μg) ) using ADORA2A antibody(31299P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    Flow cytometric analysis of 1X10^6 Hela cells labeling ADORA2A Antibody(HY-P84242
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    Immunocytochemistry analysis of U251 cells labeling ADORA2A Antibody (YA3939, Mouse) with ADORA2A Antibody (YA3939, Mouse) (HY-P84242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADORA2A Antibody (YA3939, Mouse) ((HY-P84242) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    Immunofluorescence analysis of Hela cells labeling ADORA2A antibody (HY-P84242) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    ADORA2A receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Subunit

    Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct (PubMed:16339847). May interact with DRD4 (PubMed:18832607). Interacts with NECAB2 (PubMed:17689978). Interacts (via cytoplasmic C-terminal domain) with GAS2L2; interaction enhances receptor-mediated adenylyl cyclase activity (By similarity)

  • SwissProt ID

    P29274

  • Gene ID
    135 [NCBI]
  • Synonyms

    A2aR; RDC8; ADORA2

ADORA2A Antibody (YA3939) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg) ,F9(lane 3(20μg) ,C2C12(lane 4(20μg)and 3T3(lane 5(20μg) using ADORA2A Antibody（HY-P84242）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 1(40μg) ),F9(lane 2(40μg) ),L1210(lane 3(40μg) ),C6(lane 4(40μg) ),NIH/3T3(lane 5(40μg) ),Raw264.7(lane 6(40μg) ),HepG2(lane 7(40μg) )and Hela(lane 8(40μg) ) using ADORA2A antibody(31299P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ADORA2A Antibody(HY-P84242

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U251 cells labeling ADORA2A Antibody (YA3939, Mouse) with ADORA2A Antibody (YA3939, Mouse) (HY-P84242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADORA2A Antibody (YA3939, Mouse) ((HY-P84242) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling ADORA2A antibody (HY-P84242) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • ADORA2A Antibody (YA3939) Product Image
    1/16
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • ADORA2A Antibody (YA3939) Product Image
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 1(40μg) )
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg) ,F9(lane 3(20μg) ,C2C12(lane 4(20μg)and 3T3(lane 5(20μg) using ADORA2A Antibody（HY-P84242）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 1(40μg) ),F9(lane 2(40μg) ),L1210(lane 3(40μg) ),C6(lane 4(40μg) ),NIH/3T3(lane 5(40μg) ),Raw264.7(lane 6(40μg) ),HepG2(lane 7(40μg) )and Hela(lane 8(40μg) ) using ADORA2A antibody(31299P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ADORA2A Antibody(HY-P84242
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U251 cells labeling ADORA2A Antibody (YA3939, Mouse) with ADORA2A Antibody (YA3939, Mouse) (HY-P84242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADORA2A Antibody (YA3939, Mouse) ((HY-P84242) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling ADORA2A antibody (HY-P84242) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • Experimental Validation Results for ADORA2A Antibody (YA3939)
  • ADORA2A Antibody (YA3939) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293(lane 1(40μg) )

  • ADORA2A Antibody (YA3939) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20μg) ,F9(lane 3(20μg) ,C2C12(lane 4(20μg)and 3T3(lane 5(20μg) using ADORA2A Antibody（HY-P84242）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • ADORA2A Antibody (YA3939) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293(lane 1(40μg) ),F9(lane 2(40μg) ),L1210(lane 3(40μg) ),C6(lane 4(40μg) ),NIH/3T3(lane 5(40μg) ),Raw264.7(lane 6(40μg) ),HepG2(lane 7(40μg) )and Hela(lane 8(40μg) ) using ADORA2A antibody(31299P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ADORA2A Antibody (YA3939) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling ADORA2A Antibody(HY-P84242

  • ADORA2A Antibody (YA3939) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U251 cells labeling ADORA2A Antibody (YA3939, Mouse) with ADORA2A Antibody (YA3939, Mouse) (HY-P84242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADORA2A Antibody (YA3939, Mouse) ((HY-P84242) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ADORA2A Antibody (YA3939) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling ADORA2A antibody (HY-P84242) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • ADORA2A Antibody (YA3939) Product Image WB
  • ADORA2A Antibody (YA3939) Product Image WB
  • ADORA2A Antibody (YA3939) Product Image WB
  • ADORA2A Antibody (YA3939) Product Image FC
  • ADORA2A Antibody (YA3939) Product Image ICC
  • ADORA2A Antibody (YA3939) Product Image ICC