MELK Antibody (YA1423)
(Synonyms: MELK; KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK)MELK Antibody (YA1423) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MELK.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
MELK Antibody (YA1423) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MELK.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 75 kDa
A synthesized peptide derived from human MELK aa1-36.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MELK (Maternal embryonic leucine zipper kinase) belongs to the CAMK serine/threonine protein kinase superfamily. Melk is a protein serine/threonine kinase that is maximally active during mitosis. It is involved in diverse functions such as cell cycle, cytokinesis, mRNA splicing and apoptosis. Expression MELK is expressed in cells of various tissue origins. MELK expression is strongly dependant on cell-cycle: MELK is undetectable in cells which have exited cell cycle. The exact function of MELK is currently unknown, however MELK was shown to be involved in cell cycle progression via the protein phosphatase CDC25B phosphorylation, in cytokinesis, in apoptosis via its interaction with the Bcl-2 family of proapoptotic genes and apoptosis signal-regulating kinase (ASK1) and in inhibition of mRNA splicing during mitosis via its association with NIPP1. MELK function is required for mammary tumorigenesis in vivo.
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Subcellular Localization
Cell membrane; Peripheral membrane protein
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Expression
Tissue_specificity:It is expressed in the placenta, kidneys, thymus, testes, ovaries, and intestines.
Induction:Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5-FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo -
Isoforms & Post-Translational Modification
Q14680 has 8 isomers: Q14680-1: 74642 Da (predicted); Q14680-2: 66399 Da (predicted); Q14680-3: 52528 Da (predicted); Q14680-4: 59576 Da (predicted); Q14680-5: 66547 Da (predicted); Q14680-6: 71174 Da (predicted); Q14680-7: 70150 Da (predicted); Q14680-8: 69116 Da (predicted).
Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable) -
Subunit
Monomer. Interacts with ZNF622 and PPP1R8
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SwissProt ID
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Synonyms
MELK; KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK
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Research Field
Signal Transduction
Documentation
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