FHIT Antibody
(Synonyms: Endonuclease G mitochondrial; Endo G)FHIT Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to FHIT.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
FHIT Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to FHIT.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 20 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 16 kDa
Synthetic peptide corresponding to the center region of human FHIT.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FHIT is a Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'-phosphosulfate resulting in the formation of adenosine 5'-phosphoramidate. Also catalyzes the ammonolysis of adenosine 5-phosphorofluoridate and diadenosine triphosphate. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as a tumor suppressor (By similarity)[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cytoplasm; Mitochondrion; Nucleus
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Expression
Tissue_Specificity: Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. -
Isoforms & Post-Translational Modification
FHIT has an amino acid length of 147, molecular weight is 16858 Da.SRC 对第 114 位酪氨酸(Tyr-114)的磷酸化是诱导细胞凋亡所必需的FHIT 的氨基酸个数为 147 个，分子量为 16858 Da。Phosphorylation at Tyr-114 by SRC is required for induction of apoptosis
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
Endonuclease G mitochondrial; Endo G
Documentation
[1]. Trapasso F, et al. Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding. Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1592-7. [Content Brief]
[2]. Huang K, et al. The mechanism of action of the fragile histidine triad, Fhit: isolation of a covalent adenylyl enzyme and chemical rescue of H96G-Fhit. Biochemistry. 2004 Jun 15;43(23):7637-42. [Content Brief]
[3]. Barnes LD, et al. Fhit, a putative tumor suppressor in humans, is a dinucleoside 5',5"'-P1,P3-triphosphate hydrolase. Biochemistry. 1996 Sep 10;35(36):11529-35. [Content Brief]
[4]. Lima CD, et al. Structure-based analysis of catalysis and substrate definition in the HIT protein family. Science. 1997 Oct 10;278(5336):286-90. [Content Brief]
[5]. Brenner C, et al. Purification and crystallization of complexes modeling the active state of the fragile histidine triad protein. Protein Eng. 1997 Dec;10(12):1461-3. [Content Brief]
[6]. Pace HC, et al. Genetic, biochemical, and crystallographic characterization of Fhit-substrate complexes as the active signaling form of Fhit. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5484-9. [Content Brief]
[7]. Guranowski A, et al. Fhit proteins can also recognize substrates other than dinucleoside polyphosphates. FEBS Lett. 2008 Sep 3;582(20):3152-8. [Content Brief]
[8]. Wojdyła-Mamoń AM, et al. Adenylylsulfate-ammonia adenylyltransferase activity is another inherent property of Fhit proteins. Biosci Rep. 2015 Jun 25;35(4):. [Content Brief]
[9]. Weiske J, et al. The tumor suppressor Fhit acts as a repressor of beta-catenin transcriptional activity. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20344-9. [Content Brief]
[10]. Semba S, et al. Fhit modulation of the Akt-survivin pathway in lung cancer cells: Fhit-tyrosine 114 (Y114) is essential. Oncogene. 2006 May 11;25(20):2860-72. [Content Brief]
[11]. Nishizaki M, et al. Synergistic tumor suppression by coexpression of FHIT and p53 coincides with FHIT-mediated MDM2 inactivation and p53 stabilization in human non-small cell lung cancer cells. Cancer Res. 2004 Aug 15;64(16):5745-52. [Content Brief]
[12]. Rimessi A, et al. Intramitochondrial calcium regulation by the FHIT gene product sensitizes to apoptosis. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12753-8. [Content Brief]
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