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Semaphorin 4D Antibody (YA1922)

(Synonyms: CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G; A8 antibody; BB 18 antibody; Coll 4 antibody; COLL4 antibody; Collapsin 4 antibody; GR3 antibody; M sema G antibody; MSEMA antibody; Sema 4d antibody; Sema H antibody; SEMA J antibody; Sema4d antibody; SEMAJ antibody; Semaphorin 4D antibody; Semaphorin C like 2 antibody; Semaphorin H antibody; Semaphorin J antibody; Semaphorin-4D antibody; SemaphorinJ antibody; Semcl 2 antibody; Semcl2 antibody)
Customer Review

Based on 1 Customer Validation

Semaphorin 4D Antibody (YA1922) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Semaphorin 4D.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Semaphorin 4D Antibody (YA1922)
  • Semaphorin 4D Antibody (YA1922) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Semaphorin 4D Antibody (YA1922) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Semaphorin 4D.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 96 kDa
Species Reactivity Database

Entrez Gene: 10507 Human

SwissProt: Q92854 Human

Immunogen

A synthetic peptide of human Semaphorin 4D aa142-156.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Semaphorin 4D Antibody (YA1922)
    Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Daudi (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using SEMA4D Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Semaphorin 4D cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:This gene is highly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus, and also expressed at lower levels in the testes, brain, kidneys, small intestine, prostate, heart, placenta, lungs, and pancreas, but is not expressed in the colon and liver.

  • Isoforms & Post-Translational Modification

    Q92854 has 2 isomers: Q92854-1: 96150 Da (predicted); Q92854-2: 82164 Da (predicted).

  • Subunit

    Homodimer (PubMed:20877282). Interacts with PLXNB1 (PubMed:10520995)

  • SwissProt ID

    Q92854

  • Gene ID
    10507 [NCBI]
  • Synonyms

    CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G; A8 antibody; BB 18 antibody; Coll 4 antibody; COLL4 antibody; Collapsin 4 antibody; GR3 antibody; M sema G antibody; MSEMA antibody; Sema 4d antibody; Sema H antibody; SEMA J antibody; Sema4d antibody; SEMAJ antibody; Semaphorin 4D antibody; Semaphorin C like 2 antibody; Semaphorin H antibody; Semaphorin J antibody; Semaphorin-4D antibody; SemaphorinJ antibody; Semcl 2 antibody; Semcl2 antibody

  • Research Field

    Immunology

Semaphorin 4D Antibody (YA1922) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Daudi (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using SEMA4D Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Semaphorin 4D Antibody (YA1922) Product Image
    1/16
  • Experimental Validation Results for Semaphorin 4D Antibody (YA1922)
  • Semaphorin 4D Antibody (YA1922) Product Image
  • Experimental Validation Results for Semaphorin 4D Antibody (YA1922)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Daudi (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using SEMA4D Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Semaphorin 4D Antibody (YA1922)
  • Semaphorin 4D Antibody (YA1922) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Daudi (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using SEMA4D Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Semaphorin 4D Antibody (YA1922) Product Image WB