SMG1 Antibody (YA1987)(PBS only)

Cat. No.: HY-P82242A: Data Sheet User Guide for Antibodies
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SMG1 Antibody (YA1987) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMG1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SMG1 Antibody (YA1987) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMG1.

Host

Rabbit

Molecular Weight

Predicted band size: 411 kDa;

Observed band size: 411 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96Q15

Gene ID

23049 [NCBI]

Immunogen

A synthesized peptide derived from human Smg1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver

Isoforms & Post-Translational Modification：Q96Q15 has 4 isomers: Q96Q15-1: 410501 Da (predicted); Q96Q15-2: 395382 Da (predicted); Q96Q15-3: 340674 Da (predicted); Q96Q15-4: 269425 Da (predicted).
Autophosphorylated

Subunit：Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain (PubMed:33205750). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Part of a complex composed of SMG1, DHX34 and UPF1; within the complex DHX34 acts as a scaffolding protein to facilitate SMG1 phosphorylation of UPF1 (PubMed:26841701). Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2 (PubMed:33205750). Interacts with UPF2. Interacts with DHX34 (via C-terminus); the interaction is RNA-independent (PubMed:25220460, PubMed:33205750)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

61E3.4; ATX; hSMG1; LIP; smg1

Documentation

User Guide for Antibodies (1077 KB)