SMG1 Antibody (YA1987)(PBS only)
(Synonyms: 61E3.4; ATX; hSMG1; LIP; smg1)SMG1 Antibody (YA1987) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMG1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000-1:5000 |
Product Details
SMG1 Antibody (YA1987) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMG1.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 411 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 411 kDa
A synthesized peptide derived from human Smg1
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SMG1 is a Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver -
Isoforms & Post-Translational Modification
Q96Q15 has 4 isomers: Q96Q15-1: 410501 Da (predicted); Q96Q15-2: 395382 Da (predicted); Q96Q15-3: 340674 Da (predicted); Q96Q15-4: 269425 Da (predicted).
Autophosphorylated -
Subunit
Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain (PubMed:33205750). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Part of a complex composed of SMG1, DHX34 and UPF1; within the complex DHX34 acts as a scaffolding protein to facilitate SMG1 phosphorylation of UPF1 (PubMed:26841701). Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2 (PubMed:33205750). Interacts with UPF2. Interacts with DHX34 (via C-terminus); the interaction is RNA-independent (PubMed:25220460, PubMed:33205750)
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SwissProt ID
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Synonyms
61E3.4; ATX; hSMG1; LIP; smg1
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Research Field
Epigenetics and Nuclear Signaling
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