Copy Link Print Information

Nesprin1 Antibody (YA1369)

(Synonyms: ARCA1; C6orf98; CPG2; EDMD4; Enaptin; Myne-1; MYNE1; Nesp1; Nesprin-1; SCAR8; Syne-1; SYNE1)
Customer Review

Based on 1 Customer Validation

Nesprin1 Antibody (YA1369) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Nesprin1 Antibody (YA1369) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

Nesprin1 Antibody (YA1369) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 112 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 1011 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Nesprin 1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103734

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Nesprin1 Antibody (HY-P81624) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    Immunocytochemistry analysis of C2C12 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated Nesprin1 (HY-P81624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    Immunocytochemistry analysis of A549 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nesprin1 (HY-P81624) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Nesprin1 is a Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenesis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette

  • Subcellular Localization

    Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side; Nucleus; Nucleus envelope; Cytoplasm, cytoskeleton; Cytoplasm, myofibril, sarcomere; Golgi apparatus

  • Expression


    Tissue_specificity:Expressed in HeLa, A431, A172 and HaCaT cells (at protein level) . Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, peripheral blood leukocytes, pancreas, cerebellum, stomach, kidney and placenta. Isoform GSRP-56 is predominantly expressed in heart and skeletal muscle (at protein level)

  • Isoforms & Post-Translational Modification

    Q8NF91 has 12 isomers: Q8NF91-1: 1011086 Da (predicted); Q8NF91-2: 380360 Da (predicted); Q8NF91-3: 112393 Da (predicted); Q8NF91-4: 1005239 Da (predicted); Q8NF91-5: 167250 Da (predicted); Q8NF91-6: 199267 Da (predicted); Q8NF91-7: 642672 Da (predicted); Q8NF91-8: 367873 Da (predicted); Q8NF91-9: 111583 Da (predicted); Q8NF91-10: 349010 Da (predicted); Q8NF91-11: 131185 Da (predicted); Q8NF91-12: 54914 Da (predicted).
    The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces

  • Subunit

    Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. At least SUN1/2-containing core LINC complexes are proposed to be hexameric composed of three protomers of each KASH and SUN domain-containing protein. The SUN2:SYNE1/KASH1 LINC complex is a heterohexamer; the homotrimeric cloverleave-like conformation of the SUN domain is a prerequisite for LINC complex formation in which three separate SYNE1/KASH1 peptides bind at the interface of adjacent SUN domains. Self-associates. Interacts with SYNE3. Interacts with SPAG4/SUN4. May interact with MUSK. Interacts with F-actin via its N-terminal domain. Interacts with EMD and LMNA in vitro. Interacts (via KASH domain) with TMEM258 (PubMed:28716842)

  • SwissProt ID

    Q8NF91

  • Gene ID
    23345 [NCBI]
  • Synonyms

    ARCA1; C6orf98; CPG2; EDMD4; Enaptin; Myne-1; MYNE1; Nesp1; Nesprin-1; SCAR8; Syne-1; SYNE1

  • Research Field

    Tags & Cell Markers

Nesprin1 Antibody (YA1369) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Nesprin1 Antibody (HY-P81624) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated Nesprin1 (HY-P81624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nesprin1 (HY-P81624) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Nesprin1 Antibody (YA1369) Product Image
    1/16
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Nesprin1 Antibody (YA1369) Product Image
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Nesprin1 Antibody (HY-P81624) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated Nesprin1 (HY-P81624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nesprin1 (HY-P81624) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Experimental Validation Results for Nesprin1 Antibody (YA1369)
  • Nesprin1 Antibody (YA1369) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Nesprin1 Antibody (HY-P81624) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Nesprin1 Antibody (YA1369) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of C2C12 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated Nesprin1 (HY-P81624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Nesprin1 Antibody (YA1369) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nesprin1 (HY-P81624) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Nesprin1 Antibody (YA1369) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Nesprin1 Antibody (YA1369) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Nesprin1 Antibody (YA1369) Product Image WB
  • Nesprin1 Antibody (YA1369) Product Image ICC
  • Nesprin1 Antibody (YA1369) Product Image ICC
  • Nesprin1 Antibody (YA1369) Product Image IHC-P
  • Nesprin1 Antibody (YA1369) Product Image IHC-P