Nesprin1 Antibody (YA1369)

Cat. No.: HY-P81624: Data Sheet COA
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Nesprin1 Antibody (YA1369) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin1.

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Nesprin1 Antibody (YA1369) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Nesprin1 Antibody (YA1369) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 1011 kDa;

Observed band size: 112 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8NF91

Gene ID

23345 [NCBI]

Immunogen

A synthetic peptide of human Nesprin 1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB ICC IHC-P

Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Nesprin1 Antibody (HY-P81624) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of C2C12 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated Nesprin1 (HY-P81624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A549 cells labeling Nesprin1 with Nesprin1Antibody (HY-P81624) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nesprin1 (HY-P81624) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Nesprin1 Antibody (YA1369). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenesis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette

Subcellular Localization：Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side; Nucleus; Nucleus envelope; Cytoplasm, cytoskeleton; Cytoplasm, myofibril, sarcomere; Golgi apparatus

Expression：
Tissue_specificity:Expressed in HeLa, A431, A172 and HaCaT cells (at protein level) . Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, peripheral blood leukocytes, pancreas, cerebellum, stomach, kidney and placenta. Isoform GSRP-56 is predominantly expressed in heart and skeletal muscle (at protein level)

Isoforms & Post-Translational Modification：Q8NF91 has 12 isomers: Q8NF91-1: 1011086 Da (predicted); Q8NF91-2: 380360 Da (predicted); Q8NF91-3: 112393 Da (predicted); Q8NF91-4: 1005239 Da (predicted); Q8NF91-5: 167250 Da (predicted); Q8NF91-6: 199267 Da (predicted); Q8NF91-7: 642672 Da (predicted); Q8NF91-8: 367873 Da (predicted); Q8NF91-9: 111583 Da (predicted); Q8NF91-10: 349010 Da (predicted); Q8NF91-11: 131185 Da (predicted); Q8NF91-12: 54914 Da (predicted).
The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces

Subunit：Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. At least SUN1/2-containing core LINC complexes are proposed to be hexameric composed of three protomers of each KASH and SUN domain-containing protein. The SUN2:SYNE1/KASH1 LINC complex is a heterohexamer; the homotrimeric cloverleave-like conformation of the SUN domain is a prerequisite for LINC complex formation in which three separate SYNE1/KASH1 peptides bind at the interface of adjacent SUN domains. Self-associates. Interacts with SYNE3. Interacts with SPAG4/SUN4. May interact with MUSK. Interacts with F-actin via its N-terminal domain. Interacts with EMD and LMNA in vitro. Interacts (via KASH domain) with TMEM258 (PubMed:28716842)

RRID

AB_3103734

Database

Entrez Gene: 23345 Human ; 64009 Mouse ; 499010 Rat

SwissProt: Q8NF91 Human ; Q6ZWR6 Mouse ;

OMIM: 610743 Human

Research Field

Tags & Cell Markers

Synonyms

ARCA1; C6orf98; CPG2; EDMD4; Enaptin; Myne-1; MYNE1; Nesp1; Nesprin-1; SCAR8; Syne-1; SYNE1

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Nesprin1 Antibody (YA1369) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nesprin1 Antibody (YA1369)

Nesprin1 Antibody (YA1369) Featured Recommendations:

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Nesprin1 Antibody (YA1369) Related Classifications

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