MAD2L1 Antibody
(Synonyms: MAD2L1, MAD2, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1)MAD2L1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MAD2L1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), 0.05% NaN3, 40% Glycerol
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-100 |
Product Details
MAD2L1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MAD2L1.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 24 kDa;
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 24 kDa
Fusion protein of human MAD2L1
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), 0.05% NaN3, 40% Glycerol
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MAD2L1 is a Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle. The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint. The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition. When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby reactivating APC/C for transition into anaphase. In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Chromosome, centromere, kinetochore; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole
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Isoforms & Post-Translational Modification
MAD2L1 has 2 isoforms, Q13257-1: amino acid length is 205, molecular weight is 23510 Da (predicted); Q13257-2: amino acid length is 90, molecular weight is 10335 Da (predicted).
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2. -
Subunit
Monomer and homodimer.
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SwissProt ID
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Synonyms
MAD2L1, MAD2, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1
Documentation
[1]. Luo X, et al. Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. Nat Struct Biol. 2000 Mar;7(3):224-9. [Content Brief]
[2]. Luo X, et al. The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20. Mol Cell. 2002 Jan;9(1):59-71. [Content Brief]
[3]. Luo X, et al. The Mad2 spindle checkpoint protein has two distinct natively folded states. Nat Struct Mol Biol. 2004 Apr;11(4):338-45. [Content Brief]
[4]. Ji W, et al. Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling. J Biol Chem. 2018 Jan 12;293(2):484-496. [Content Brief]
[5]. Alfieri C, et al. Molecular basis of APC/C regulation by the spindle assembly checkpoint. Nature. 2016 Aug 25;536(7617):431-436. [Content Brief]
[6]. Johnson VL, et al. Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression. J Cell Sci. 2004 Mar 15;117(Pt 8):1577-89. [Content Brief]
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