NEDD4 Antibody (YA7690)
(Synonyms: KIAA0093, NEDD4-1, RPF1, PIG53, NEDD4, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4)Based on 1 Customer Validation
NEDD4 Antibody (YA7690) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEDD4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-100 | 1:50-200 | 1:20-50 |
Product Details
NEDD4 Antibody (YA7690) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEDD4.
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Host Rabbit
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Clonality Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 149 kDa
A synthesized peptide derived from human NEDD4: 1271-1319.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NEDD4 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to the direct link between NEDD4 and PTEN regulation through polyubiquitination described in is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Ubiquitinates DAZAP2, leading to its proteasomal degradation. Ubiquitinates POLR2A. Functions as a platform to recruit USP13 to form an NEDD4-USP13 deubiquitination complex that plays a critical role in cleaving the 'Lys-48'-linked ubiquitin chains of VPS34 and then stabilizing VPS34, thus promoting the formation of autophagosomes |(Microbial infection) Involved in the ubiquitination of Ebola virus protein VP40 which plays a role in viral budding[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cytoplasm,Nucleus,Cell membrane
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Isoforms & Post-Translational Modification
P46934 has four isomers: P46934-1: 149114 Da (predicted); P46934-2: 147384 Da (predicted); P46934-3: 140696 Da (predicted); P46934-4: 104217 Da (predicted).
Undergoes 'Lys-29'-linked auto-ubiquitination at Lys-1279 and serves as a scaffold for recruiting USP13 to form an NEDD4-USP13 deubiquitination complex -
Subunit
Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif
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SwissProt ID
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Synonyms
KIAA0093, NEDD4-1, RPF1, PIG53, NEDD4, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4
Documentation
[1]. Harreman M, et al. Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation. Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20705-10. [Content Brief]
[2]. Maspero E, et al. Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation. EMBO Rep. 2011 Apr;12(4):342-9. [Content Brief]
[3]. Maspero E, et al. Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming. Nat Struct Mol Biol. 2013 Jun;20(6):696-701. [Content Brief]
[4]. Persaud A, et al. Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function. EMBO J. 2011 Jul 15;30(16):3259-73. [Content Brief]
[5]. Pham N, et al. Nedd4 regulates ubiquitination and stability of the guanine-nucleotide exchange factor CNrasGEF. J Biol Chem. 2001 Dec 14;276(50):46995-7003. [Content Brief]
[6]. Fouladkou F, et al. The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization. Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8585-90. [Content Brief]
[7]. Wang X, et al. NEDD4-1 is a proto-oncogenic ubiquitin ligase for PTEN. Cell. 2007 Jan 12;128(1):129-39. [Content Brief]
[8]. Lin Q, et al. HECT E3 ubiquitin ligase Nedd4-1 ubiquitinates ACK and regulates epidermal growth factor (EGF)-induced degradation of EGF receptor and ACK. Mol Cell Biol. 2010 Mar;30(6):1541-54. [Content Brief]
[9]. Low LH, et al. Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response. J Biol Chem. 2015 Mar 13;290(11):7141-50. [Content Brief]
[10]. Hamilton MH, et al. Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates. J Biol Chem. 2001 Jul 13;276(28):26324-31. [Content Brief]
[11]. Xie W, et al. Auto-ubiquitination of NEDD4-1 Recruits USP13 to Facilitate Autophagy through Deubiquitinating VPS34. Cell Rep. 2020 Feb 25;30(8):2807-2819.e4. [Content Brief]
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