HCFC1 Antibody
(Synonyms: HCF1, HFC1, HCFC1, Host cell factor 1, HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory protein)HCFC1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to HCFC1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
HCFC1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to HCFC1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 100-170 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 208 kDa
Synthetic peptide corresponding to the N-term region of human HCFC1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HCFC1 is a Transcriptional coregulator (By similarity). Serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators. Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Recruits KMT2E/MLL5 to E2F1 responsive promoters promoting transcriptional activation and thereby facilitates G1 to S phase transition. Modulates expression of homeobox protein PDX1, perhaps acting in concert with transcription factor E2F1, thereby regulating pancreatic beta-cell growth and glucose-stimulated insulin secretion (By similarity). May negatively modulate transcriptional activity of FOXO3 (By similarity)[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_Specificity: Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested. -
Isoforms & Post-Translational Modification
HCFC1 has 4 isoforms, P51610-1: amino acid length is 2035, molecular weight is 208732 Da (predicted); P51610-2: amino acid length is 1966, molecular weight is 201850 Da (predicted); P51610-3: amino acid length is 428, molecular weight is 47189 Da (predicted); P51610-4: amino acid length is 2079, molecular weight is 213405 Da (predicted).会在 HCF 重复序列内的一个或多个 PPCE--THET 位点发生蛋白水解切割(PubMed:10920196, PubMed:21285374, PubMed:7590226)。最初切割产生的 N 端和 C 端肽链会被进一步切割，发生“修剪”并积累更短的肽链。O-糖基化可促进切割过程(PubMed:21285374, PubMed:28302723, PubMed:28584052)HCFC1 存在 4 个异构体，P51610-1：氨基酸个数为 2035 个，分子量为 208732 Da (预测)；P51610-2：氨基酸个数为 1966 个，分子量为 201850 Da (预测)；P51610-3：氨基酸个数为 428 个，分子量为 47189 Da (预测)；P51610-4：氨基酸个数为 2079 个，分子量为 213405 Da (预测)。Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats (PubMed:10920196, PubMed:21285374, PubMed:7590226). Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains. Cleavage is promoted by O-glycosylation (PubMed:21285374, PubMed:28302723, PubMed:28584052)
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Subunit
Composed predominantly of six polypeptides ranging from 110 to 150 kDa and a minor 300 kDa polypeptide.
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SwissProt ID
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Synonyms
HCF1, HFC1, HCFC1, Host cell factor 1, HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory protein
Documentation
[1]. Vinckevicius A, et al. Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin. Mol Cell Biol. 2015 Dec;35(24):4135-46. [Content Brief]
[2]. Mahajan SS, et al. Mutations in host cell factor 1 separate its role in cell proliferation from recruitment of VP16 and LZIP. Mol Cell Biol. 2000 Feb;20(3):919-28. [Content Brief]
[3]. Scarr RB, et al. A novel 50-kilodalton fragment of host cell factor 1 (C1) in G(0) cells. Mol Cell Biol. 2000 May;20(10):3568-75. [Content Brief]
[4]. Khurana B, et al. A protein sequestering system reveals control of cellular programs by the transcriptional coactivator HCF-1. J Biol Chem. 2004 Aug 6;279(32):33673-83. [Content Brief]
[5]. Vogel JL, et al. Site-specific proteolysis of the transcriptional coactivator HCF-1 can regulate its interaction with protein cofactors. Proc Natl Acad Sci U S A. 2006 May 2;103(18):6817-22. [Content Brief]
[6]. Zhou P, et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem. 2013 Jun 14;288(24):17532-43. [Content Brief]
[7]. Vogel JL, et al. The novel coactivator C1 (HCF) coordinates multiprotein enhancer formation and mediates transcription activation by GABP. EMBO J. 2000 Feb 15;19(4):683-90. [Content Brief]
[8]. Piluso D, et al. Host cell factor-1 interacts with and antagonizes transactivation by the cell cycle regulatory factor Miz-1. J Biol Chem. 2002 Nov 29;277(48):46799-808. [Content Brief]
[9]. Luciano RL, et al. HCF-1 functions as a coactivator for the zinc finger protein Krox20. J Biol Chem. 2003 Dec 19;278(51):51116-24. [Content Brief]
[10]. Wysocka J, et al. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes Dev. 2003 Apr 1;17(7):896-911. [Content Brief]
[11]. Mazars R, et al. The THAP-zinc finger protein THAP1 associates with coactivator HCF-1 and O-GlcNAc transferase: a link between DYT6 and DYT3 dystonias. J Biol Chem. 2010 Apr 30;285(18):13364-71. [Content Brief]
[12]. Cai Y, et al. Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. J Biol Chem. 2010 Feb 12;285(7):4268-72. [Content Brief]
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