Glucose 6 Phosphate Dehydrogenase Antibody (YA411)

Cat. No.: HY-P80688: Data Sheet COA
SDS
User Guide for Antibodies
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Glucose 6 Phosphate Dehydrogenase Antibody (YA411) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 59 kDa;

Observed band size: 59 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P11413

Gene ID

2539 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Glucose 6 Phosphate Dehydrogenase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:200-1:500
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and C212 (lane 3) and C6 (lane 4) using Glucose 6 Phosphate Dehydrogenase antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis

Subcellular Localization：Cytoplasm, cytosol; Membrane; Peripheral membrane protein

Expression：
Tissue_specificity:The long subtype is found in lymphoblasts, granulocytes, and sperm.

Isoforms & Post-Translational Modification：P11413 has 3 isomers: P11413-1: 59257 Da (predicted); P11413-2: 63827 Da (predicted); P11413-3: 62468 Da (predicted).
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity

Subunit：Homotetramer; dimer of dimers (PubMed:10745013, PubMed:15858258, PubMed:24769394, PubMed:38066190). Interacts with SIRT2; the interaction is enhanced by H(2)O(2) treatment (PubMed:24769394). Forms a ternary complex with ALDOB and TP53; this interaction is direct. ALDOB stabilizes the complex inhibiting G6PD activity and keeping oxidative pentose phosphate metabolism in check

RRID

AB_3102667

Database

Entrez Gene: 2539 Human ; 14381 Mouse ; 24377 Rat

SwissProt: P11413 Human ; Q00612 Mouse ; P05370 Rat

OMIM: 300908 Human

Research Field

Signal Transduction

Synonyms

G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Glucose 6 Phosphate Dehydrogenase Antibody (YA411)

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Featured Recommendations:

Product Detail

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Background

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Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Related Classifications

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