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Lck Antibody (YA712)

(Synonyms: LCK; Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK)
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Based on 1 Customer Validation

Lck Antibody (YA712) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lck.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Lck Antibody (YA712)
  • Lck Antibody (YA712) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Lck Antibody (YA712) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lck.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database

Entrez Gene: 3932 Human

SwissProt: P06239 Human

Immunogen

Synthetic peptide corresponding to Human LCK.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102689

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Lck Antibody (YA712)
    Western blot analysis of extracts from Ramos (lane 2, 20ug), Raji (lane 3, 20ug), Jurkat (lane 4, 20ug) and Hela (lane 5, 20ug) using Lck Antibody (HY-P80743). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Lck (lymphocyte-specific protein tyrosine kinase) is a Src-family non-receptor tyrosine kinase that functions as a central initiator of T-cell activation and antigen receptor signaling through phosphorylation-dependent intracellular signal transduction mechanisms[1][2]. Mechanistically, Lck operates at the proximal stage of the T-cell receptor (TCR) signaling pathway, where it phosphorylates immunoreceptor tyrosine-based activation motifs (ITAMs) and promotes downstream signaling events required for T-cell activation, differentiation, and immune responses[2][3]. Genetic studies demonstrated that loss of functional Lck disrupts TCR-mediated signaling and impairs cellular responsiveness, establishing its essential role in lymphocyte biology and experimental models of immune deficiency[4]. Disease relevance is supported by evidence linking LCK deficiency to severe immunological dysfunction, while dysregulated Lck signaling has also been investigated in hematologic malignancies and other immune-associated disorders[1][4]. Compared with related Src-family kinases, particularly Fyn, Lck has a more dominant role in initiating proximal TCR signaling, whereas Fyn contributes to overlapping but nonessential signaling functions in many T-cell activation contexts[3][5]. Structurally, Lck contains SH2, SH3, and catalytic kinase domains that coordinate activation-state regulation through phosphorylation-dependent conformational changes[2]. For experimental applications, Lck remains a widely used target for kinase inhibition studies and mechanistic analyses of T-cell signaling networks, making it a valuable tool for immune signaling research and therapeutic target validation[6].

  • Subcellular Localization

    Cell membrane; Lipid-anchor; Cytoplasmic side; Cytoplasm, cytosol

  • Expression


    Tissue_specificity:Specifically expressed in lymphocytes

  • Isoforms & Post-Translational Modification

    P06239 has 3 isomers: P06239-1: 58001 Da (predicted); P06239-2: 40866 Da (predicted); P06239-3: 61190 Da (predicted).
    Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. Dephosphorylation at Tyr-394 by DUSP22 negatively regulates T-cell receptor signaling (PubMed:24714587, PubMed:38225265);Myristoylation is required prior to palmitoylation;Palmitoylation regulates association with the plasma membrane and could be mediated by ZDHHC2;'Lys-63'-linked ubiquitinated at Lys-99 and Lys-276 by UBR2; this modification is required for autophosphorylation at Tyr-394

  • Subunit

    Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB1 and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B and PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocyte migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylation in the presence of functional ZAP70. Interacts with UNC119; this interaction plays a crucial role in activation of LCK. Interacts with CEACAM1 (via cytoplasmic domain); mediates CEACAM1 phosphorylation resulting in PTPN6 recruitment that dephosphorylates TCR stimulation-induced CD247 and ZAP70 (PubMed:18424730). Interacts with CD160. Interacts with CD48 (PubMed:12007789)

  • SwissProt ID

    P06239

  • Gene ID
    3932 [NCBI]
  • Synonyms

    LCK; Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK

  • Research Field

    Immunology

[1]. Uniprotkb.

[2]. Rossy J, et al. How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism. Front Immunol. 2012 Jun 19;3:167. [Content Brief]

[3]. Lck gene information from NCBI.

[4]. Straus DB, et al. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell. 1992 Aug 21;70(4):585-93. [Content Brief]

[5]. Wikipedia.

[6]. Elkamhawy A, et al. New horizons in drug discovery of lymphocyte-specific protein tyrosine kinase (Lck) inhibitors: a decade review (2011-2021) focussing on structure-activity relationship (SAR) and docking insights. J Enzyme Inhib Med Chem. 2021 Dec;36(1):1574-1602. [Content Brief]

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Lck Antibody (YA712) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Ramos (lane 2, 20ug), Raji (lane 3, 20ug), Jurkat (lane 4, 20ug) and Hela (lane 5, 20ug) using Lck Antibody (HY-P80743). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Lck Antibody (YA712) Product Image
    1/16
  • Experimental Validation Results for Lck Antibody (YA712)
  • Lck Antibody (YA712) Product Image
  • Experimental Validation Results for Lck Antibody (YA712)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Ramos (lane 2, 20ug), Raji (lane 3, 20ug), Jurkat (lane 4, 20ug) and Hela (lane 5, 20ug) using Lck Antibody (HY-P80743). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Lck Antibody (YA712)
  • Lck Antibody (YA712) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Ramos (lane 2, 20ug), Raji (lane 3, 20ug), Jurkat (lane 4, 20ug) and Hela (lane 5, 20ug) using Lck Antibody (HY-P80743). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Lck Antibody (YA712) Product Image WB